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Home  >  ELISA Kit  >  Human ACACA ELISA Kit
ACACA (GeneName), Acetyl-CoA carboxylase 1 (ProteinName), ACACA_HUMAN.
Product Name:

Human ACACA/ Acetyl-CoA carboxylase 1 ELISA Kit

Cat.#:

E1284h

Brand:
EIAab®
Regulatory Status:
Alternative:

ACC1, ACAC, ACC1, ACCA, Acetyl-Coenzyme A carboxylase alpha, ACC-alpha

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
32.1-2000pg/mL
Sensitivity:
15.7pg/mL
Specificity:
Natural and recombinant human Acetyl-CoA carboxylase 1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Metabolism
Human ACACA ELISA Kit
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Human ACACA ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.5%

Recovery
Recovery was determined by spiking various levels of Acetyl-CoA carboxylase 1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

97

91-103

Plasma

99

93-105

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acetyl-CoA carboxylase 1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

99-110%

99-108%

95-106%

108-118%

EDTA plasma(n=5)

86-96%

108-116%

98-109%

114-124%

heparin plasma(n=5)

109-120%

 

97-97%

88-96%

90-98%

 

General Annotation


Sub Unit:
Monomer, homodimer, and homotetramer (PubMed:20952656). Can form filamentous polymers (PubMed:20457939, PubMed:20952656). Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis (PubMed:12360400, PubMed:16326698). Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity (PubMed:20457939).


Function:
Catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA, a key metabolite in the fatty acid synthetic (PubMed:20952656, PubMed:20457939). Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase (PubMed:20952656, PubMed:20457939).


Subcellular Location:
Cytoplasm


This product has not yet been referenced specifically in any publications.

[1].
"Acetyl-CoA carboxylase alpha gene and breast cancer susceptibility."

[2].
"Acetyl-coenzyme A carboxylase alpha gene variations may be associated with the direct effects of some antipsychotics on triglyceride levels."

[3].
"Haplotype-based analysis of common variation in the acetyl-coA carboxylase alpha gene and breast cancer risk: a case-control study nested within the European Prospective Investigation into Cancer and Nutrition."

[4].
"BRCA1 affects lipid synthesis through its interaction with acetyl-CoA carboxylase."

[5].
"Asymmetric expression of transcripts derived from the shared promoter between the divergently oriented ACACA and TADA2L genes."

[6].
"Acetyl-CoA carboxylase alpha gene and breast cancer susceptibility."

[7].
"Acetyl-coenzyme A carboxylase alpha gene variations may be associated with the direct effects of some antipsychotics on triglyceride levels."

[8].
"Haplotype-based analysis of common variation in the acetyl-coA carboxylase alpha gene and breast cancer risk: a case-control study nested within the European Prospective Investigation into Cancer and Nutrition."

[9].
"BRCA1 affects lipid synthesis through its interaction with acetyl-CoA carboxylase."

[10].
"Asymmetric expression of transcripts derived from the shared promoter between the divergently oriented ACACA and TADA2L genes."
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