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Abp1 (GeneName), Amiloride-sensitive amine oxidase [copper-containing] (ProteinName), ABP1_RAT.
Product Name:

Rat Abp1/ Amiloride-sensitive amine oxidase [copper-containing] ELISA Kit

Cat.#:

E0656r

Brand:
EIAab®
Regulatory Status:
Alternative:

DAO, Diamine oxidase, Amiloride-binding protein, ABP, Histaminase

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
3.12-200ng/mL
Sensitivity:
1.66ng/mL
Specificity:
Natural and recombinant rat Amiloride-sensitive amine oxidase [copper-containing]
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Signal Transduction
Rat Abp1 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Recovery
Recovery was determined by spiking various levels of Amiloride-sensitive amine oxidase [copper-containing] into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

99

93-105

Plasma

101

95-107

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amiloride-sensitive amine oxidase [copper-containing] and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

106-116%

92-102%

82-92%

83-92%

EDTA plasma(n=5)

105-114%

83-93%

86-86%

109-117%

heparin plasma(n=5)

85-97%

 

93-105%

88-98%

100-108%

 

General Annotation


Sub Unit:
Homodimer; disulfide-linked.


Function:
Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis.


Subcellular Location:
Secreted extracellular space


This product has not yet been referenced specifically in any publications.
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