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ABP1 (GeneName), Amiloride-sensitive amine oxidase [copper-containing] (ProteinName), ABP1_HUMAN.
Product Name:

Human ABP1/ Amiloride-sensitive amine oxidase [copper-containing] ELISA Kit

Cat.#:

E0656h

Brand:
EIAab®
Regulatory Status:
Alternative:

DAO, Diamine oxidase, Amiloride-binding protein, ABP, Histaminase, Kidney amine oxidase, KAO, AOC1, DAO1

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100ng/mL
Sensitivity:
0.56ng/mL
Specificity:
Natural and recombinant human Amiloride-sensitive amine oxidase [copper-containing]
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Signal Transduction
Human ABP1 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.5%

Recovery
Recovery was determined by spiking various levels of Amiloride-sensitive amine oxidase [copper-containing] into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

93

87-99

Plasma

95

89-101

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amiloride-sensitive amine oxidase [copper-containing] and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

99-109%

92-103%

101-110%

106-115%

EDTA plasma(n=5)

101-113%

106-116%

96-107%

82-92%

heparin plasma(n=5)

100-111%

 

108-118%

83-92%

101-111%

 

General Annotation


Sub Unit:
Homodimer; disulfide-linked.


Function:
Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function.


Subcellular Location:
Secreted extracellular space


This product has not yet been referenced specifically in any publications.
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