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ABP1 (GeneName), Amiloride-sensitive amine oxidase [copper-containing] (ProteinName), ABP1_HUMAN.

Product Name:

Human ABP1/ Amiloride-sensitive amine oxidase [copper-containing] ELISA Kit

Cat.#:

E0656h

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

DAO, Diamine oxidase, Amiloride-binding protein, ABP, Histaminase, Kidney amine oxidase, KAO, AOC1, DAO1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/mL

Sensitivity:

0.56ng/mL

Specificity:

Natural and recombinant human Amiloride-sensitive amine oxidase [copper-containing]

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Signal Transduction

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Human ABP1 ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations
Sequence / 3D
Feedback Wall (0)

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.5%

Recovery

Recovery was determined by spiking various levels of Amiloride-sensitive amine oxidase [copper-containing] into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	93	87-99
Plasma	95	89-101

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amiloride-sensitive amine oxidase [copper-containing] and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	99-109%	92-103%	101-110%	106-115%
EDTA plasma(n=5)	101-113%	106-116%	96-107%	82-92%
heparin plasma(n=5)	100-111%	108-118%	83-92%	101-111%

General Annotation

Sub Unit:

Homodimer; disulfide-linked.

Function:

Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. Placental DAO is thought to play a role in the regulation of the female reproductive function.

Subcellular Location:

Secreted extracellular space

Database link

Uniprot:

P19801

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