ABCB9 (GeneName), ATP-binding cassette sub-family B member 9 (ProteinName), ABCB9_HUMAN.
Product Name:
Human ABCB9/ ATP-binding cassette sub-family B member 9 ELISA Kit
Cat.#:
E1181h
Brand:
EIAab®
Regulatory Status:
Alternative:
ATP-binding cassette transporter 9, ABC transporter 9 protein, TAP-like protein, TAPL, KIAA1520
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
40.6pg/mL
Specificity:
Natural and recombinant human ATP-binding cassette sub-family B member 9
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:

Research Area:
-
- Data
- Citations
- Publication
- Sequence / 3D
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.0%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤6.3%
Recovery
Recovery was determined by spiking various levels of ATP-binding cassette sub-family B member 9 into serum and plasma.
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
95 |
89-101 |
Plasma |
97 |
91-103 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATP-binding cassette sub-family B member 9 and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
110-119% |
93-102% |
92-101% |
102-113% |
EDTA plasma(n=5) |
105-116% |
106-116% |
108-118% |
108-118% |
heparin plasma(n=5) |
86-97%
|
94-104% |
94-104% |
107-119% |
General Annotation
Sub Unit:
Homodimer.
Function:
ATP-dependent low-affinity peptide transporter which translocates a broad spectrum of peptides from the cytosol to the lysosomal lumen. Displays a broad peptide length specificity from 6-mer up to at least 59-mer peptides with an optimum of 23-mers. Favors positively charged, aromatic or hydrophobic residues in the N- and C-terminal positions whereas negatively charged residues as well as asparagine and methionine are not favored.
Subcellular Location:
Lysosome membrane
Multi-pass membrane protein
May be located in membrane rafts.
This product has not yet been referenced specifically in any publications.
[1].
"Biochemical characterization of transporter associated with antigen processing (TAP)-like (ABCB9) expressed in insect cells."
[2].
"Peptide specificity and lipid activation of the lysosomal transport complex ABCB9 (TAPL)."
[4].
"Selective and ATP-dependent translocation of peptides by the homodimeric ATP binding cassette transporter TAP-like (ABCB9)."
[5].
"Membrane localization of transporter associated with antigen processing (TAP)-like (ABCB9) visualized in vivo with a fluorescence protein-fusion technique."
[6].
"Gene organization of human transporter associated with antigen processing-like (TAPL, ABCB9): analysis of alternative splicing variants and promoter activity."
[7].
"Characterization of ABCB9, an ATP binding cassette protein associated with lysosomes."
[8].
"Transporter associated with antigen processing-like (ABCB9) stably expressed in Chinese hamster ovary-K1 cells is sorted to the microdomains of lysosomal membranes."
[9].
"Duffy-null-associated low neutrophil counts influence HIV-1 susceptibility in high-risk South African black women."
[10].
"Tuning the cellular trafficking of the lysosomal peptide transporter TAPL by its N-terminal domain."
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