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ABCB9 (GeneName), ATP-binding cassette sub-family B member 9 (ProteinName), ABCB9_HUMAN.
Product Name:

Human ABCB9/ ATP-binding cassette sub-family B member 9 ELISA Kit

Cat.#:

E1181h

Brand:
EIAab®
Regulatory Status:
Alternative:

ATP-binding cassette transporter 9, ABC transporter 9 protein, TAP-like protein, TAPL, KIAA1520

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
40.6pg/mL
Specificity:
Natural and recombinant human ATP-binding cassette sub-family B member 9
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human ABCB9 ELISA Kit
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Human ABCB9 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.0%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.3%

Recovery
Recovery was determined by spiking various levels of ATP-binding cassette sub-family B member 9 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

95

89-101

Plasma

97

91-103

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATP-binding cassette sub-family B member 9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

110-119%

93-102%

92-101%

102-113%

EDTA plasma(n=5)

105-116%

106-116%

108-118%

108-118%

heparin plasma(n=5)

86-97%

 

94-104%

94-104%

107-119%

 

General Annotation


Sub Unit:
Homodimer.


Function:
ATP-dependent low-affinity peptide transporter which translocates a broad spectrum of peptides from the cytosol to the lysosomal lumen. Displays a broad peptide length specificity from 6-mer up to at least 59-mer peptides with an optimum of 23-mers. Favors positively charged, aromatic or hydrophobic residues in the N- and C-terminal positions whereas negatively charged residues as well as asparagine and methionine are not favored.


Subcellular Location:
Lysosome membrane Multi-pass membrane protein May be located in membrane rafts.


This product has not yet been referenced specifically in any publications.

[1].
"Biochemical characterization of transporter associated with antigen processing (TAP)-like (ABCB9) expressed in insect cells."

[2].
"Peptide specificity and lipid activation of the lysosomal transport complex ABCB9 (TAPL)."

[3].
"Functional dissection of transmembrane domains of human TAP-like (ABCB9)."

[4].
"Selective and ATP-dependent translocation of peptides by the homodimeric ATP binding cassette transporter TAP-like (ABCB9)."

[5].
"Membrane localization of transporter associated with antigen processing (TAP)-like (ABCB9) visualized in vivo with a fluorescence protein-fusion technique."

[6].
"Gene organization of human transporter associated with antigen processing-like (TAPL, ABCB9): analysis of alternative splicing variants and promoter activity."

[7].
"Characterization of ABCB9, an ATP binding cassette protein associated with lysosomes."

[8].
"Transporter associated with antigen processing-like (ABCB9) stably expressed in Chinese hamster ovary-K1 cells is sorted to the microdomains of lysosomal membranes."

[9].
"Duffy-null-associated low neutrophil counts influence HIV-1 susceptibility in high-risk South African black women."

[10].
"Tuning the cellular trafficking of the lysosomal peptide transporter TAPL by its N-terminal domain."
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