ABCB5 (GeneName), ATP-binding cassette sub-family B member 5 (ProteinName), ABCB5_HUMAN.
Product Name:
Human ABCB5/ ATP-binding cassette sub-family B member 5 ELISA Kit
Cat.#:
E10410h
Brand:
EIAab®
Regulatory Status:
Alternative:
ABCB5 P-gp, P-glycoprotein ABCB5
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.098ng/mL
Specificity:
Natural and recombinant human ATP-binding cassette sub-family B member 5
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Cancer
- Data
- Citations
- Publication
- Sequence / 3D
- Feedback Wall (0)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.1%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤6.8%
Recovery
Recovery was determined by spiking various levels of ATP-binding cassette sub-family B member 5 into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
85 |
80-91 |
|
Plasma |
87 |
81-93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATP-binding cassette sub-family B member 5 and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
84-97% |
104-114% |
80-91% |
86-96% |
|
EDTA plasma(n=5) |
99-108% |
100-109% |
92-101% |
101-110% |
|
heparin plasma(n=5) |
103-115%
|
110-120% |
90-99% |
105-117% |
General Annotation
Sub Unit:
N/A
Function:
Drug efflux transporter present in a number of stem cells that acts as a regulator of cellular differentiation. Able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.
Subcellular Location:
Cell membrane
Multi-pass membrane protein
Database link
UniGene:
SMR:
STRING:
KEGG:
MIM:
Pfam:
Uniprot:
Valid Sequence:
This product has not yet been referenced specifically in any publications.
[1].
"Regulation of progenitor cell fusion by ABCB5 P-glycoprotein, a novel human ATP-binding cassette transporter."
[2].
"ABCB5 maintains melanoma-initiating cells through a proinflammatory cytokine signaling circuit."
[3].
"ABCB5 is a limbal stem cell gene required for corneal development and repair."
[4].
"Genetically determined ABCB5 functionality correlates with pigmentation phenotype and melanoma risk."
Feedback Wall
Displaying result : 0
All customer
All customer
Default sort
Default sort
Newest first
Oldest first
No feedback yet, let's comment.
Message
Product Name *
Email *
Message: *
Inform
Size
Quantity
Price ($)
Subtotal 1 ($)
Subtotal 2:
$
Size
Quantity
Price ($)
Discount:
Please select at least one unit!
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:
Fax:
Email:sales@eiaab.com
Please contact us:
Tel:
Fax:
Email:sales@eiaab.com