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Abca4 (基因名), Retinal-specific phospholipid-transporting ATPase ABCA4 (蛋白名), ABCA4_MOUSE.

产品名称:

Mouse Abca4/ Retinal-specific phospholipid-transporting ATPase ABCA4 ELISA Kit
视网膜特异性磷脂转运atp酶ABCA4

货号:

E10399m

商标:

EIAab^®

监管等级:

RUO

别名:

ATP-binding cassette sub-family A member 4, RIM ABC transporter, RIM protein, Abcr, Retinal-specific ATP-binding cassette transporter

检测方法:

ELISA

实验类型:

Sandwich

检测范围:

0.156-10ng/mL

灵敏度:

0.078ng/mL

特异性:

Natural and recombinant mouse Retinal-specific phospholipid-transporting ATPase ABCA4

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

实验步骤:

研究领域:

规格 & 价格: 登录.

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Mouse Abca4 ELISA Kit

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产品说明书

数据表:

说明书:

MSDS:

数据
文献
出版物
序列 / 3D
常见问题解答
反馈墙 (0)

通用注释

亚单元:

N/A

功能:

Catalyzes the translocation of specific phospholipids from the extracellular/lumenal to the cytoplasmic leaflet of membrane coupled to the hydrolysis of ATP. Transports preferentially phosphatidylethanolamine. In the visual cycle, acts as an inward-directed retinoid flipase, retinoid substrates imported by ABCA4 from the extracellular or intradiscal (rod) membrane surfaces to the cytoplasmic membrane surface are all-trans-retinaldehyde (ATR) and N-retinyl-phosphatidyl-ethanolamine (NR-PE). Once transported to the cytoplasmic surface, ATR is reduced to vitamin A by trans-retinol dehydrogenase (tRDH) and then transferred to the retinal pigment epithelium (RPE) where it is converted to 11-cis-retinal. May play a role in photoresponse, removing ATR/NR-PE from the extracellular photoreceptor surfaces during bleach recovery.

亚细胞位置:

Membrane Multi-pass membrane protein Endoplasmic reticulum Localized to outer segment disk edges of rods and cones, with around one million copies/photoreceptor.

数据库链接

UniGene:

Mm.3918

SMR:

O35600

STRING:

10090.ENSMUSP00000013995

KEGG:

mmu:11304

Pfam:

PF00005

Uniprot:

O35600

相关产品

Human ABCA4 ELISA Kit

Human ABCA4 CLIA Kit

Human ABCA4 Protein

Human ABCA4 Polyclonal Antibody

Human ABCA4 Monoclonal Antibody

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AGASWQAKLE | EKSGRLQ TQ | EPLPAGSEQL | ANGSNPTAAE | DKHTRSPQ

3D 模型:

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该产品尚未在任何出版物中被引用。

[1].

"Molecular analysis of the ABCA4 gene for reliable detection of allelic variations in Spanish patients: identification of 21 novel variants."

(PMID: 19028736)

[2].

"Late-onset Stargardt disease is associated with missense mutations that map outside known functional regions of ABCR (ABCA4)."

(PMID: 11379881)

[3].

"Variation of codons 1961 and 2177 of the Stargardt disease gene is not associated with age-related macular degeneration."

(PMID: 11346402)

[4].

"An analysis of allelic variation in the ABCA4 gene."

(PMID: 11328725)

[5].

"Frequency of ABCA4 mutations in 278 Spanish controls: an insight into the prevalence of autosomal recessive Stargardt disease."

(PMID: 18977788)

[6].

"Genetic and epigenetic factors at COL2A1 and ABCA4 influence clinical outcome in congenital toxoplasmosis."

(PMID: 18523590)

[7].

"ABCA4 gene analysis in patients with autosomal recessive cone and cone rod dystrophies."

(PMID: 18285826)

[8].

"N965S is a common ABCA4 variant in Stargardt-related retinopathies in the Danish population."

(PMID: 17982420)

[9].

"Spectrum of the ABCA4 gene mutations implicated in severe retinopathies in Spanish patients."

(PMID: 17325136)

[10].

"Microarray-based mutation analysis of the ABCA4 (ABCR) gene in autosomal recessive cone-rod dystrophy and retinitis pigmentosa."

(PMID: 15494742)

[1].

小鼠视网膜特异性磷脂转运atp酶ABCA4(Abca4)ELISA试剂盒可以做多少个样本？

小鼠视网膜特异性磷脂转运atp酶ABCA4(Abca4)ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠视网膜特异性磷脂转运atp酶ABCA4(Abca4)ELISA试剂盒使用视频？

小鼠视网膜特异性磷脂转运atp酶ABCA4(Abca4)ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠视网膜特异性磷脂转运atp酶ABCA4(Abca4)ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠视网膜特异性磷脂转运atp酶ABCA4(Abca4)ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠视网膜特异性磷脂转运atp酶ABCA4(Abca4)ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠视网膜特异性磷脂转运atp酶ABCA4(Abca4)ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠视网膜特异性磷脂转运atp酶ABCA4(Abca4)ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠视网膜特异性磷脂转运atp酶ABCA4(Abca4)ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠视网膜特异性磷脂转运atp酶ABCA4(Abca4)ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠视网膜特异性磷脂转运atp酶ABCA4(Abca4)ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠视网膜特异性磷脂转运atp酶ABCA4(Abca4)ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠视网膜特异性磷脂转运atp酶ABCA4(Abca4)ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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Mouse Abca4/ Retinal-specific phospholipid-transporting ATPase ABCA4 ELISA Kit 视网膜特异性磷脂转运atp酶ABCA4

E10399m

ATP-binding cassette sub-family A member 4, RIM ABC transporter, RIM protein, Abcr, Retinal-specific ATP-binding cassette transporter

Mouse Abca4/ Retinal-specific phospholipid-transporting ATPase ABCA4 ELISA Kit
视网膜特异性磷脂转运atp酶ABCA4