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APOBEC3G (基因名), DNA dC->dU-editing enzyme APOBEC-3G (蛋白名), ABC3G_HUMAN.

产品名称:

Human APOBEC3G/ DNA dC->dU-editing enzyme APOBEC-3G ELISA Kit

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

APOBEC-related cytidine deaminase, APOBEC-related protein, APOBEC-related protein 9, ARP-9, CEM-15, CEM15, Deoxycytidine deaminase, A3G, MDS019

检测方法:

ELISA

特异性:

Natural and recombinant human DNA dC->dU-editing enzyme APOBEC-3G

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

规格 & 价格: 登录.

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产品说明书

数据
文献
出版物
序列 / 3D
常见问题解答
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通用注释

亚单元:

Homodimer. Homooligomer. Can bind RNA to form ribonucleoprotein complexes of high-molecular-mass (HMM) or low-molecular-mass (LMM). HMM is inactive and heterogeneous in protein composition because of binding nonselectively to cellular RNAs, which in turn are associated with variety of cellular proteins. The LMM form which is enzymatically active has few or no RNAs associated. Its ability to form homooligomer is distinct from its ability to assemble into HMM. Interacts with APOBEC3B, APOBEC3F, MOV10, AGO2, EIF4E, EIF4ENIF1, DCP2 and DDX6 in an RNA-dependent manner. Interacts with AGO1, AGO3 and PKA/PRKACA. Interacts with HIV-1 VIF and reverse transcriptase/ribonuclease H. Interacts with hepatitis B virus capsid protein.

功能:

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits potent antiviral activity against vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single-or double-stranded RNA. Exhibits antiviral activity also against simian immunodeficiency viruses (SIVs), hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV). May inhibit the mobility of LTR and non-LTR retrotransposons.

亚细胞位置:

Cytoplasm Nucleus Cytoplasm P-body Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection, virion-encapsidated in absence of HIV-1 VIF.

数据库链接

UniGene:

Hs.660143

SMR:

Q9HC16

STRING:

9606.ENSP00000385057

KEGG:

hsa:60489

MIM:

607113

Pfam:

PF08210

Uniprot:

Q9HC16

相关产品

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Human GDF5 ELISA Kit

序列长度:

384

序列:

50:

100:

150:

200:

250:

300:

350:

384:

VDHQGCPFQP | WDGLDEHSQD | LSGRLRAILQ | NQEN

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"APOBEC3G expression is dysregulated in primary HIV-1 infection and polymorphic variants influence CD4+ T-cell counts and plasma viral load."

(PMID: 19996938)

[2].

"Structure, interaction and real-time monitoring of the enzymatic reaction of wild-type APOBEC3G."

(PMID: 19153609)

[3].

"Structure of the DNA deaminase domain of the HIV-1 restriction factor APOBEC3G."

(PMID: 18288108)

[4].

"[The associations between polymorphisms of APOBEC3G and different outcomes of persistent HBV infection]."

(PMID: 18647522)

[5].

"Absence of H186R polymorphism in exon 4 of the APOBEC3G gene among North Indian individuals."

(PMID: 18652534)

[6].

"Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif."

(PMID: 18836454)

[7].

"Crystal structure of the anti-viral APOBEC3G catalytic domain and functional implications."

(PMID: 18849968)

[8].

"Restriction of foamy viruses by APOBEC cytidine deaminases."

(PMID: 16378963)

[9].

"Exhaustive genotyping of the CEM15 (APOBEC3G) gene and absence of association with AIDS progression in a French cohort."

(PMID: 15609224)

[10].

"A single amino acid substitution in human APOBEC3G antiretroviral enzyme confers resistance to HIV-1 virion infectivity factor-induced depletion."

(PMID: 15054139)

[1].

人APOBEC3GELISA试剂盒可以做多少个样本？

人APOBEC3GELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人APOBEC3GELISA试剂盒使用视频？

人APOBEC3GELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人APOBEC3GELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人APOBEC3GELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人APOBEC3GELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人APOBEC3GELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人APOBEC3GELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人APOBEC3GELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人APOBEC3GELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人APOBEC3GELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人APOBEC3GELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人APOBEC3GELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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