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Apbb1ip (基因名), Amyloid beta A4 precursor protein-binding family B member 1-interacting protein (蛋白名), AB1IP_MOUSE.

产品名称:

Mouse Apbb1ip/ Amyloid beta A4 precursor protein-binding family B member 1-interacting protein ELISA Kit

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

APBB1-interacting protein 1, Proline-rich EVH1 ligand 1, PREL-1, Proline-rich protein 48, Prel1

检测方法:

ELISA

特异性:

Natural and recombinant mouse Amyloid beta A4 precursor protein-binding family B member 1-interacting protein

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

规格 & 价格: 登录.

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产品说明书

数据
文献
出版物
序列 / 3D
常见问题解答
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通用注释

亚单元:

Interacts, through the N-terminal Pro-rich region, with the WW domain of APBB1. Interacts with RAP1A, PFN1, VASP and ENAH.

功能:

Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion.

亚细胞位置:

Cell membrane Peripheral membrane protein Cell projection Lamellipodium Cell junction Focal adhesion Cytoplasm Cytoskeleton Colocalizes with ENA/VASP proteins at lamellipodia tips and focal adhesions, and F-actin at the leading edge. At the membrane surface, associates, via the PH domain, preferentially with the inositol phosphates, PtdIns(5)P and PtdIns(3)P. This binding appears to be necessary for the efficient interaction of the RA domain to Ras-GTPases.

数据库链接

UniGene:

Mm.14255

SMR:

Q8R5A3

STRING:

10090.ENSMUSP00000014290

KEGG:

mmu:54519

Pfam:

PF00169

Uniprot:

Q8R5A3

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Human GDF5 ELISA Kit

序列长度:

670

序列:

50:

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670:

EQDFMSDLMK | ALQKKRGNIP |

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"RIAM, an Ena/VASP and Profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion."

(PMID: 15469846)

[2].

"The retinoic acid-responsive proline-rich protein is identified in promyeloleukemic HL-60 cells."

(PMID: 14530287)

[3].

"A genome-wide association study of inflammatory biomarker changes in response to fenofibrate treatment in the Genetics of Lipid Lowering Drug and Diet Network."

(PMID: 22228203)

[4].

"Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score."

(PMID: 20379614)

[5].

"Association studies of 23 positional/functional candidate genes on chromosome 10 in late-onset Alzheimer's disease."

(PMID: 17373700)

[6].

"A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease."

(PMID: 16385451)

[7].

"PREL1 provides a link from Ras signalling to the actin cytoskeleton via Ena/VASP proteins."

(PMID: 15642358)

[8].

"The DNA sequence and comparative analysis of human chromosome 10."

(PMID: 15164054)

[9].

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

(PMID: 15489334)

[10].

"The WW domain of neural protein FE65 interacts with proline-rich motifs in Mena, the mammalian homolog of Drosophila enabled."

(PMID: 9407065)

[1].

小鼠Apbb1ipELISA试剂盒可以做多少个样本？

小鼠Apbb1ipELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠Apbb1ipELISA试剂盒使用视频？

小鼠Apbb1ipELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠Apbb1ipELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠Apbb1ipELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠Apbb1ipELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠Apbb1ipELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠Apbb1ipELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠Apbb1ipELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠Apbb1ipELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠Apbb1ipELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠Apbb1ipELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠Apbb1ipELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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