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Got2 (GeneName), Aspartate aminotransferase, mitochondrial (ProteinName), AATM_MOUSE.
Product Name:

Mouse Got2/ Aspartate aminotransferase, mitochondrial ELISA Kit

Cat.#:

E1612m

Brand:
EIAab®
Regulatory Status:
Alternative:

mAspAT, Fatty acid-binding protein, FABP-1, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, FABPpm, Transaminase A, Got-2

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50U/L
Sensitivity:
0.39U/L
Specificity:
Natural and recombinant mouse Aspartate aminotransferase, mitochondrial
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cell Biology
Mouse Got2 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.1%

Recovery
Recovery was determined by spiking various levels of Aspartate aminotransferase, mitochondrial into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

103

97-109

Plasma

105

99-111

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aspartate aminotransferase, mitochondrial and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

90-100%

106-116%

106-118%

97-107%

EDTA plasma(n=5)

81-90%

109-119%

106-118%

108-118%

heparin plasma(n=5)

105-115%

 

88-100%

91-101%

94-107%

 

General Annotation


Sub Unit:
Homodimer.


Function:
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.


Subcellular Location:
Mitochondrion matrix Cell membrane


This product has not yet been referenced specifically in any publications.

[1].
"Genome-wide joint SNP and CNV analysis of aortic root diameter in African Americans: the HyperGEN study."

[2].
"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."

[3].
"Common variants at ten loci influence QT interval duration in the QTGEN Study."

[4].
"Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging."

[5].
"Mitochondrial aspartate aminotransferase: a third kynurenate-producing enzyme in the mammalian brain."

[6].
"Complete sequencing and characterization of 21,243 full-length human cDNAs."

[7].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

[8].
"Ethanol up-regulates fatty acid uptake and plasma membrane expression and export of mitochondrial aspartate aminotransferase in HepG2 cells."

[9].
"Nucleotide sequence and tissue distribution of the human mitochondrial aspartate aminotransferase mRNA."

[10].
"The primary structure of mitochondrial aspartate aminotransferase from human heart."
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