Got2 (GeneName), Aspartate aminotransferase, mitochondrial (ProteinName), AATM_MOUSE.
Product Name:
Mouse Got2/ Aspartate aminotransferase, mitochondrial ELISA Kit
Cat.#:
E1612m
Brand:
EIAab®
Regulatory Status:
Alternative:
mAspAT, Fatty acid-binding protein, FABP-1, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, FABPpm, Transaminase A, Got-2
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50U/L
Sensitivity:
0.39U/L
Specificity:
Natural and recombinant mouse Aspartate aminotransferase, mitochondrial
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Cell Biology
- Data
- Citations
- Publication
- Sequence / 3D
- Feedback Wall (0)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤9.1%
Recovery
Recovery was determined by spiking various levels of Aspartate aminotransferase, mitochondrial into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
103 |
97-109 |
|
Plasma |
105 |
99-111 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aspartate aminotransferase, mitochondrial and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
90-100% |
106-116% |
106-118% |
97-107% |
|
EDTA plasma(n=5) |
81-90% |
109-119% |
106-118% |
108-118% |
|
heparin plasma(n=5) |
105-115%
|
88-100% |
91-101% |
94-107% |
General Annotation
Sub Unit:
Homodimer.
Function:
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.
Subcellular Location:
Mitochondrion matrix
Cell membrane
Database link
Valid Sequence:
This product has not yet been referenced specifically in any publications.
[1].
"Genome-wide joint SNP and CNV analysis of aortic root diameter in African Americans: the HyperGEN study."
[2].
"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."
[3].
"Common variants at ten loci influence QT interval duration in the QTGEN Study."
[4].
"Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging."
[5].
"Mitochondrial aspartate aminotransferase: a third kynurenate-producing enzyme in the mammalian brain."
[7].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
[8].
"Ethanol up-regulates fatty acid uptake and plasma membrane expression and export of mitochondrial aspartate aminotransferase in HepG2 cells."
[9].
"Nucleotide sequence and tissue distribution of the human mitochondrial aspartate aminotransferase mRNA."
[10].
"The primary structure of mitochondrial aspartate aminotransferase from human heart."
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