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Got1 (GeneName), Aspartate aminotransferase, cytoplasmic (ProteinName), AATC_RAT.
Product Name:

Rat Got1/ Aspartate aminotransferase, cytoplasmic ELISA Kit

Cat.#:

E1214r

Brand:
EIAab®
Regulatory Status:
Alternative:

cAspAT, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, cCAT, Glutamate oxaloacetate transaminase 1, Transaminase A

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
3.12-200U/L
Sensitivity:
1.63U/L
Specificity:
Natural and recombinant rat Aspartate aminotransferase, cytoplasmic
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cell Biology
Rat Got1 ELISA Kit
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Rat Got1 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.2%

Recovery
Recovery was determined by spiking various levels of Aspartate aminotransferase, cytoplasmic into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

97

91-103

Plasma

99

93-105

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aspartate aminotransferase, cytoplasmic and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

83-94%

110-120%

101-112%

114-126%

EDTA plasma(n=5)

105-115%

90-100%

102-112%

110-118%

heparin plasma(n=5)

97-107%

 

102-112%

100-110%

110-120%

 

General Annotation


Sub Unit:
Homodimer.


Function:
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.


Subcellular Location:
Cytoplasm



[1].
"Properties of human liver cytosolic aspartate aminotransferase mRNAs generated by alternative polyadenylation site selection."

[2].
"Genome-wide association study identifies genetic variants in GOT1 determining serum aspartate aminotransferase levels."

[3].
"Human variation in alcohol response is influenced by variation in neuronal signaling genes."

[4].
"The DNA sequence and comparative analysis of human chromosome 10."

[5].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

[6].
"Genetic homogeneity, high-resolution mapping, and mutation analysis of the urofacial (Ochoa) syndrome and exclusion of the glutamate oxaloacetate transaminase gene (GOT1) in the critical region as the disease gene."

[7].
"The amino acid sequence of cytosolic aspartate aminotransferase from human liver."

[8].
"Neither ischemic parenchymal volume nor severe grade complication correlate transient high transaminase elevation after liver resection."

[9].
"Architecture of the human interactome defines protein communities and disease networks."

[10].
"HIF-2α regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma."
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