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首页  >  酶联免疫试剂盒  >  Human GOT1 ELISA Kit
GOT1 (基因名), Aspartate aminotransferase, cytoplasmic (蛋白名), AATC_HUMAN.
产品名称:

Human GOT1/ Aspartate aminotransferase, cytoplasmic ELISA Kit
细胞质天冬氨酸转氨

货号:

E1214h

商标:
EIAab®
监管等级:
别名:

cAspAT, Cysteine aminotransferase, cytoplasmic, Cysteine transaminase, cytoplasmic, cCAT, Glutamate oxaloacetate transaminase 1, Transaminase A

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
1.56-100U/L
灵敏度:
0.87U/L
特异性:
Natural and recombinant human Aspartate aminotransferase, cytoplasmic
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Cell Biology
Human GOT1 ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤4.9%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤6.2%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

94

88-100

Plasma

96

90-102

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

93-105%

93-105%

96-105%

107-119%

EDTA plasma(n=5)

107-116%

101-111%

108-120%

91-100%

heparin plasma(n=5)

82-92%

 

109-117%

109-119%

93-103%

 

通用注释


亚单元:
Homodimer.


功能:
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.


亚细胞位置:
Cytoplasm


该产品尚未在任何出版物中被引用。

[1].
"Properties of human liver cytosolic aspartate aminotransferase mRNAs generated by alternative polyadenylation site selection."

[2].
"Genome-wide association study identifies genetic variants in GOT1 determining serum aspartate aminotransferase levels."

[3].
"Human variation in alcohol response is influenced by variation in neuronal signaling genes."

[4].
"The DNA sequence and comparative analysis of human chromosome 10."

[5].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

[6].
"Genetic homogeneity, high-resolution mapping, and mutation analysis of the urofacial (Ochoa) syndrome and exclusion of the glutamate oxaloacetate transaminase gene (GOT1) in the critical region as the disease gene."

[7].
"The amino acid sequence of cytosolic aspartate aminotransferase from human liver."

[8].
"Neither ischemic parenchymal volume nor severe grade complication correlate transient high transaminase elevation after liver resection."

[9].
"Architecture of the human interactome defines protein communities and disease networks."

[10].
"HIF-2α regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma."
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