027-59234612(+86)
EIAab
Home  >  ELISA Kit  >  Pig PRKAA1 ELISA Kit
PRKAA1 (GeneName), 5'-AMP-activated protein kinase catalytic subunit alpha-1 (ProteinName), AAPK1_PIG.
Product Name:

Pig PRKAA1/ 5'-AMP-activated protein kinase catalytic subunit alpha-1 ELISA Kit

Cat.#:

E9709p

Brand:
EIAab®
Regulatory Status:
Alternative:

AMPK subunit alpha-1, AMPK 63 kDa subunit, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, AMPK1

Detection Method:
ELISA
Assay Type:
Sandwich
Specificity:
Natural and recombinant pig 5'-AMP-activated protein kinase catalytic subunit alpha-1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Neurosciences
Pig PRKAA1 ELISA Kit
Size & Price: Sign In.
×
Pig PRKAA1 ELISA Kit
Email *
Message *
Please Sign In.
Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5'-AMP-activated protein kinase catalytic subunit alpha-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

112-120%

96-108%

110-120%

93-103%

EDTA plasma(n=5)

109-119%

105-114%

90-100%

94-103%

heparin plasma(n=5)

105-115%

 

98-111%

115-115%

105-116%

 

General Annotation


Sub Unit:
AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.


Function:
Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.


Subcellular Location:
Cytoplasm Nucleus In response to stress, recruited by p53/TP53 to specific promoters.


This product has not yet been referenced specifically in any publications.
Close
Sample Data
Close
Sample Data
Close
Sample Data
My Cart close
Attention close
History close
Clear All
Comparison close
User
My Cart
Attention
History
Comparison
Top
Message
close_message
Product Name *
Email *
Message: *
Inform
new Message
Size Quantity Price ($) Subtotal 1 ($)
Subtotal 2: $
triangle
Size Quantity Price ($)
Do you want to be our agent and get a lower discount?
Please contact us:
Tel:027-59234612(+86)
Fax:027-59759960(+86)
Email:sales@eiaab.com