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PRKAG1 (GeneName), 5'-AMP-activated protein kinase subunit gamma-1 (ProteinName), AAKG1_PIG.
Product Name:

Pig PRKAG1/ 5'-AMP-activated protein kinase subunit gamma-1 ELISA Kit

Cat.#:

E10187p

Brand:
EIAab®
Regulatory Status:
Alternative:

AMPK gamma1, 38 kDa subunit

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.054ng/mL
Specificity:
Natural and recombinant pig 5'-AMP-activated protein kinase subunit gamma-1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cardiovascular
Pig PRKAG1 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5'-AMP-activated protein kinase subunit gamma-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

107-107%

106-116%

104-114%

99-110%

EDTA plasma(n=5)

83-93%

106-116%

112-122%

98-108%

heparin plasma(n=5)

100-109%

 

104-112%

93-103%

86-94%

 

General Annotation


Sub Unit:
AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.


Function:
AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.


Subcellular Location:
N/A


This product has not yet been referenced specifically in any publications.
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