Home > ELISA Kit > Rat Prkab1 ELISA Kit

Prkab1 (GeneName), 5'-AMP-activated protein kinase subunit beta-1 (ProteinName), AAKB1_RAT.

Product Name:

Rat Prkab1/ 5'-AMP-activated protein kinase subunit beta-1 ELISA Kit

Cat.#:

E1671r

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

AMPK subunit beta-1, 5'-AMP-activated protein kinase 40 kDa subunit

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.081ng/mL

Specificity:

Natural and recombinant rat 5'-AMP-activated protein kinase subunit beta-1

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Cardiovascular

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Rat Prkab1 ELISA Kit

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Product Datasheets

Instruction:

MSDS:

Data
Citations
Sequence / 3D
Feedback Wall (0)

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Recovery

Recovery was determined by spiking various levels of 5'-AMP-activated protein kinase subunit beta-1 into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	89	83-95
Plasma	91	85-97

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5'-AMP-activated protein kinase subunit beta-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-105%	92-100%	104-114%	98-109%
EDTA plasma(n=5)	84-97%	99-108%	107-117%	100-110%
heparin plasma(n=5)	81-90%	91-100%	100-110%	107-117%

General Annotation

Sub Unit:

AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.

Function:

Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

Subcellular Location:

N/A

Database link

UniGene:

Rn.3619

SMR:

P80386

STRING:

10116.ENSRNOP00000001508

KEGG:

rno:83803

Pfam: