Prkab1 (GeneName), 5'-AMP-activated protein kinase subunit beta-1 (ProteinName), AAKB1_RAT.
Product Name:
Rat Prkab1/ 5'-AMP-activated protein kinase subunit beta-1 ELISA Kit
Cat.#:
E1671r
Brand:
EIAab®
Regulatory Status:
Alternative:
AMPK subunit beta-1, 5'-AMP-activated protein kinase 40 kDa subunit
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.081ng/mL
Specificity:
Natural and recombinant rat 5'-AMP-activated protein kinase subunit beta-1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:

Research Area:
Cardiovascular
- Data
- Citations
- Sequence / 3D
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤8.1%
Recovery
Recovery was determined by spiking various levels of 5'-AMP-activated protein kinase subunit beta-1 into serum and plasma.
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
89 |
83-95 |
Plasma |
91 |
85-97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5'-AMP-activated protein kinase subunit beta-1 and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
95-105% |
92-100% |
104-114% |
98-109% |
EDTA plasma(n=5) |
84-97% |
99-108% |
107-117% |
100-110% |
heparin plasma(n=5) |
81-90%
|
91-100% |
100-110% |
107-117% |
General Annotation
Sub Unit:
AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.
Function:
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Subcellular Location:
N/A
This product has not yet been referenced specifically in any publications.
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