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PRKAB1 (GeneName), 5'-AMP-activated protein kinase subunit beta-1 (ProteinName), AAKB1_HUMAN.
Product Name:

Human PRKAB1/ 5'-AMP-activated protein kinase subunit beta-1 ELISA Kit

Cat.#:

E1671h

Brand:
EIAab®
Regulatory Status:
Alternative:

AMPK subunit beta-1, AMPK

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.16ng/mL
Specificity:
Natural and recombinant human 5'-AMP-activated protein kinase subunit beta-1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cardiovascular
Human PRKAB1 ELISA Kit
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Human PRKAB1 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.6%

Recovery
Recovery was determined by spiking various levels of 5'-AMP-activated protein kinase subunit beta-1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

92

86-98

Plasma

94

88-100

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5'-AMP-activated protein kinase subunit beta-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

106-115%

84-97%

108-118%

113-122%

EDTA plasma(n=5)

92-102%

107-117%

108-116%

114-124%

heparin plasma(n=5)

109-120%

 

102-111%

94-105%

103-112%

 

General Annotation


Sub Unit:
AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.


Function:
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).


Subcellular Location:
N/A


This product has not yet been referenced specifically in any publications.
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