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AADAT (GeneName), Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial (ProteinName), AADAT_HUMAN.
Product Name:

Human AADAT/ Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial ELISA Kit

Cat.#:

E12537h

Brand:
EIAab®
Regulatory Status:
Alternative:

KAT/AadAT, 2-aminoadipate aminotransferase, 2-aminoadipate transaminase, Alpha-aminoadipate aminotransferase, AadAT, Kynurenine aminotransferase II, Kynurenine--oxoglutarate aminotransferase II, Kynurenine--oxoglutarate transaminase 2, Kynurenine--oxoglutarate transaminase II, KAT2

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/ml
Sensitivity:
0.1ng/ml
Specificity:
Natural and recombinant human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cell Biology
Human AADAT ELISA Kit
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Human AADAT ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.6%

Recovery
Recovery was determined by spiking various levels of Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

89

83-95

Plasma

91

85-97

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

97-108%

108-118%

84-95%

83-93%

EDTA plasma(n=5)

90-100%

109-119%

95-107%

104-114%

heparin plasma(n=5)

81-92%

 

103-115%

86-96%

96-106%

 

General Annotation


Sub Unit:
Homodimer.


Function:
Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).


Subcellular Location:
Mitochondrion


This product has not yet been referenced specifically in any publications.

[1].
"Crystal structure of human kynurenine aminotransferase II."

[2].
"Crystal structure of human kynurenine aminotransferase II, a drug target for the treatment of schizophrenia."

[3].
"Substrate specificity and structure of human aminoadipate aminotransferase/kynurenine aminotransferase II."

[4].
"Characterization of the human gene encoding alpha-aminoadipate aminotransferase (AADAT)."

[5].
"Genomic organization and expression analysis of mouse kynurenine aminotransferase II, a possible factor in the pathophysiology of Huntington's disease."

[6].
"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."

[7].
"Complete sequencing and characterization of 21,243 full-length human cDNAs."

[8].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

[9].
"2-Aminoadipate-2-oxoglutarate aminotransferase isoenzymes in human liver: a plausible physiological role in lysine and tryptophan metabolism."

[10].
"Architecture of the human interactome defines protein communities and disease networks."
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