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AADAT (GeneName), Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial (ProteinName), AADAT_HUMAN.

Product Name:

Human AADAT/ Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial ELISA Kit

Cat.#:

E12537h

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

KAT/AadAT, 2-aminoadipate aminotransferase, 2-aminoadipate transaminase, Alpha-aminoadipate aminotransferase, AadAT, Kynurenine aminotransferase II, Kynurenine--oxoglutarate aminotransferase II, Kynurenine--oxoglutarate transaminase 2, Kynurenine--oxoglutarate transaminase II, KAT2

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/ml

Sensitivity:

0.1ng/ml

Specificity:

Natural and recombinant human Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Cell Biology

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Human AADAT ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations
Publication
Sequence / 3D
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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.6%

Recovery

Recovery was determined by spiking various levels of Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	89	83-95
Plasma	91	85-97

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	97-108%	108-118%	84-95%	83-93%
EDTA plasma(n=5)	90-100%	109-119%	95-107%	104-114%
heparin plasma(n=5)	81-92%	103-115%	86-96%	96-106%

General Annotation

Sub Unit:

Homodimer.

Function:

Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).

Subcellular Location:

Mitochondrion

Database link

UniGene:

Hs.529735

SMR:

Q8N5Z0

STRING:

9606.ENSP00000226840

KEGG:

hsa:51166

MIM:

611754

Pfam:

PF00155

Uniprot:

Q8N5Z0

Related Product

Mouse Aadat ELISA Kit

Rat Aadat ELISA Kit

Bovine AADAT ELISA Kit

Mouse Aadat CLIA Kit

Rat Aadat CLIA Kit

Sequence length:

425

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

425:

SFSSASPEQM | DVAFQVLAQL | IKESL

3D Structure:

Predicted Epitope:

Valid Sequence:

This product has not yet been referenced specifically in any publications.

[1].

"Crystal structure of human kynurenine aminotransferase II."

(PMID: 18056995)

[2].

"Crystal structure of human kynurenine aminotransferase II, a drug target for the treatment of schizophrenia."

(PMID: 18056996)

[3].

"Substrate specificity and structure of human aminoadipate aminotransferase/kynurenine aminotransferase II."

(PMID: 18620547)

[4].

"Characterization of the human gene encoding alpha-aminoadipate aminotransferase (AADAT)."

(PMID: 12126930)

[5].

"Genomic organization and expression analysis of mouse kynurenine aminotransferase II, a possible factor in the pathophysiology of Huntington's disease."

(PMID: 10441733)

[6].

"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."

(PMID: 20877624)

[7].

"Complete sequencing and characterization of 21,243 full-length human cDNAs."

(PMID: 14702039)

[8].

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

(PMID: 15489334)

[9].

"2-Aminoadipate-2-oxoglutarate aminotransferase isoenzymes in human liver: a plausible physiological role in lysine and tryptophan metabolism."