SERPINA3 (GeneName), Alpha-1-antichymotrypsin (ProteinName), AACT_HUMAN.
Product Name:
Human SERPINA3/ Alpha-1-antichymotrypsin ELISA Kit
Cat.#:
E1015h
Brand:
EIAab®
Regulatory Status:
Alternative:
ACT, Cell growth-inhibiting gene 24/25 protein, Serpin A3, AACT, GIG24, GIG25
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100umol/L
Sensitivity:
0.078umol/L
Specificity:
Natural and recombinant human Alpha-1-antichymotrypsin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Neurosciences
- Data
- Citations
- Publication
- Sequence / 3D
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤5.5%
Recovery
Recovery was determined by spiking various levels of Alpha-1-antichymotrypsin into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
84 |
80-90 |
|
Plasma |
86 |
80-92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-1-antichymotrypsin and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
91-102% |
102-110% |
81-91% |
81-89% |
|
EDTA plasma(n=5) |
111-121% |
106-116% |
99-109% |
87-98% |
|
heparin plasma(n=5) |
102-111%
|
83-93% |
82-95% |
96-106% |
General Annotation
Sub Unit:
Interacts with DNAJC1.
Function:
Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
Subcellular Location:
Secreted
Database link
Valid Sequence:
This product has not yet been referenced specifically in any publications.
[1].
"Alpha-1 antichymotrypsin gene signal peptide a/t polymorphism and primary intracerebral hemorrhage."
[2].
"The alanine/threonine polymorphism of the alpha-1-antichymotrypsin (SERPINA3) gene and ruptured intracranial aneurysms in the Japanese population."
[3].
"Alpha-1-antichymotrypsin (ACT or SERPINA3) polymorphism may affect age-at-onset and disease duration of Alzheimer's disease."
[4].
"A new promoter polymorphism in the alpha-1-antichymotrypsin gene is a disease modifier of Alzheimer's disease."
[5].
"The SANT2 domain of the murine tumor cell DnaJ-like protein 1 human homologue interacts with alpha1-antichymotrypsin and kinetically interferes with its serpin inhibitory activity."
[6].
"Distribution of plasma alpha 1-antichymotrypsin levels in Alzheimer disease patients and controls and their genetic controls."
[7].
"A linkage disequilibrium between genes at the serine protease inhibitor gene cluster on chromosome 14q32.1 is associated with Wegener's granulomatosis."
[8].
"alpha-1-Antichymotrypsin gene A1252G variant (ACT Isehara-1) is associated with a lacunar type of ischemic cerebrovascular disease."
[9].
"Molecular studies define the primary structure of alpha1-antichymotrypsin (ACT) protease inhibitor in Alzheimer's disease brains. Comparison of act in hippocampus and liver."
[10].
"Arginine substitutions in the hinge region of antichymotrypsin affect serpin beta-sheet rearrangement."
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