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Home  >  ELISA Kit  >  Human SERPINA3 ELISA Kit
SERPINA3 (GeneName), Alpha-1-antichymotrypsin (ProteinName), AACT_HUMAN.
Product Name:

Human SERPINA3/ Alpha-1-antichymotrypsin ELISA Kit

Cat.#:

E1015h

Brand:
EIAab®
Regulatory Status:
Alternative:

ACT, Cell growth-inhibiting gene 24/25 protein, Serpin A3, AACT, GIG24, GIG25

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100umol/L
Sensitivity:
0.078umol/L
Specificity:
Natural and recombinant human Alpha-1-antichymotrypsin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Neurosciences
Human SERPINA3 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤5.5%

Recovery
Recovery was determined by spiking various levels of Alpha-1-antichymotrypsin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

84

80-90

Plasma

86

80-92

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-1-antichymotrypsin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

91-102%

102-110%

81-91%

81-89%

EDTA plasma(n=5)

111-121%

106-116%

99-109%

87-98%

heparin plasma(n=5)

102-111%

 

83-93%

82-95%

96-106%

 

General Annotation


Sub Unit:
Interacts with DNAJC1.


Function:
Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.


Subcellular Location:
Secreted


This product has not yet been referenced specifically in any publications.

[1].
"Alpha-1 antichymotrypsin gene signal peptide a/t polymorphism and primary intracerebral hemorrhage."

[2].
"The alanine/threonine polymorphism of the alpha-1-antichymotrypsin (SERPINA3) gene and ruptured intracranial aneurysms in the Japanese population."

[3].
"Alpha-1-antichymotrypsin (ACT or SERPINA3) polymorphism may affect age-at-onset and disease duration of Alzheimer's disease."

[4].
"A new promoter polymorphism in the alpha-1-antichymotrypsin gene is a disease modifier of Alzheimer's disease."

[5].
"The SANT2 domain of the murine tumor cell DnaJ-like protein 1 human homologue interacts with alpha1-antichymotrypsin and kinetically interferes with its serpin inhibitory activity."

[6].
"Distribution of plasma alpha 1-antichymotrypsin levels in Alzheimer disease patients and controls and their genetic controls."

[7].
"A linkage disequilibrium between genes at the serine protease inhibitor gene cluster on chromosome 14q32.1 is associated with Wegener's granulomatosis."

[8].
"alpha-1-Antichymotrypsin gene A1252G variant (ACT Isehara-1) is associated with a lacunar type of ischemic cerebrovascular disease."

[9].
"Molecular studies define the primary structure of alpha1-antichymotrypsin (ACT) protease inhibitor in Alzheimer's disease brains. Comparison of act in hippocampus and liver."

[10].
"Arginine substitutions in the hinge region of antichymotrypsin affect serpin beta-sheet rearrangement."
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