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Adora1 (GeneName), Adenosine receptor A1 (ProteinName), AA1R_MOUSE.
Product Name:

Mouse Adora1/ Adenosine receptor A1 ELISA Kit

Cat.#:

E12273m

Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
39pg/mL
Specificity:
Natural and recombinant mouse Adenosine receptor A1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Mouse Adora1 ELISA Kit
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Mouse Adora1 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤8.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.4%

Recovery
Recovery was determined by spiking various levels of Adenosine receptor A1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

102

96-108

Plasma

104

98-110

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Adenosine receptor A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

102-111%

103-113%

84-93%

99-108%

EDTA plasma(n=5)

105-116%

107-119%

100-108%

96-106%

heparin plasma(n=5)

98-108%

 

91-101%

105-114%

98-108%

 

General Annotation


Sub Unit:
N/A


Function:
Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.


Subcellular Location:
Cell membrane Multi-pass membrane protein


This product has not yet been referenced specifically in any publications.

[1].
"Cloning and functional characterization of a human A1 adenosine receptor."

[2].
"Mutation in ADORA1 identified as likely cause of early-onset parkinsonism and cognitive dysfunction."

[3].
"Association study of 182 candidate genes in anorexia nervosa."

[4].
"Physiogenomic analysis of statin-treated patients: domain-specific counter effects within the ACACB gene on low-density lipoprotein cholesterol?"

[5].
"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

[6].
"Contribution of adenosine related genes to the risk of depression with disturbed sleep."

[7].
"Adenosine A1 receptor gene variants associated with post-traumatic seizures after severe TBI."

[8].
"Physiogenomic comparison of edema and BMI in patients receiving rosiglitazone or pioglitazone."

[9].
"Follow-up of a major linkage peak on chromosome 1 reveals suggestive QTLs associated with essential hypertension: GenNet study."

[10].
"Adenosine deaminase and adenosine receptor polymorphisms in aspirin-intolerant asthma."
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