首页 > 酶联免疫试剂盒 > Human A2ML1 ELISA Kit

A2ML1 (基因名), Alpha-2-macroglobulin-like protein 1 (蛋白名), A2ML1_HUMAN.

产品名称:

Human A2ML1/ Alpha-2-macroglobulin-like protein 1 ELISA Kit

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

C3 and PZP-like alpha-2-macroglobulin domain-containing protein 9, CPAMD9

检测方法:

ELISA

特异性:

Natural and recombinant human Alpha-2-macroglobulin-like protein 1

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

规格 & 价格: 登录.

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产品说明书

数据
文献
出版物
序列 / 3D
常见问题解答
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通用注释

亚单元:

Monomer.

功能:

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity). Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases.

亚细胞位置:

Secreted

数据库链接

UniGene:

Hs.620532

STRING:

9606.ENSP00000299698

KEGG:

hsa:144568

MIM:

610627

Pfam:

PF00207

Uniprot:

A8K2U0

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Human GDF5 ELISA Kit

序列长度:

1454

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DPCE

3D 模型:

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免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"A novel protease inhibitor of the alpha2-macroglobulin family expressed in the human epidermis."

(PMID: 16298998)

[2].

"Transcriptomic and genetic studies identify IL-33 as a candidate gene for Alzheimer's disease."

(PMID: 19204726)

[3].

"Complete sequencing and characterization of 21,243 full-length human cDNAs."

(PMID: 14702039)

[4].

"Architecture of the human interactome defines protein communities and disease networks."

(PMID: 28514442)

[5].

"Association Between Brain Gene Expression, DNA Methylation, and Alteration of Ex Vivo Magnetic Resonance Imaging Transverse Relaxation in Late-Life Cognitive Decline."

(PMID: 29084334)

[6].

"Genetic and Environmental Determinants of Otitis Media in an Indigenous Filipino Population."

(PMID: 27484237)

[7].

"Middle ear microbiome differences in indigenous Filipinos with chronic otitis media due to a duplication in the A2ML1 gene."

(PMID: 27799062)

[8].

"Heterozygous germline mutations in A2ML1 are associated with a disorder clinically related to Noonan syndrome."

(PMID: 24939586)

[9].

"Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes."

(PMID: 25609649)

[10].

"External ear anomalies and hearing impairment in Noonan Syndrome."

(PMID: 25862627)

[1].

人A2ML1ELISA试剂盒可以做多少个样本？

人A2ML1ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

人A2ML1ELISA试剂盒使用视频？

人A2ML1ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

人A2ML1ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的人A2ML1ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

人A2ML1ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

人A2ML1ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析人A2ML1ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

人A2ML1ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集人A2ML1ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

人A2ML1ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

人A2ML1ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

人A2ML1ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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