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A2m (GeneName), Alpha-2-macroglobulin (ProteinName), A2MG_RAT.

Product Name:

Rat A2m/ Alpha-2-macroglobulin ELISA Kit

Cat.#:

E1017r

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

Alpha-2-M, A2m1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

7.8-500ng/ml

Sensitivity:

4.02ng/mL

Specificity:

Natural and recombinant rat Alpha-2-macroglobulin

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

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Rat A2m ELISA Kit

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Product Datasheets

Instruction:

MSDS:

Data
Citations
Publication
Sequence / 3D
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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.8%

Recovery

Recovery was determined by spiking various levels of Alpha-2-macroglobulin into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-103
Plasma	99	93-105

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-2-macroglobulin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	98-109%	81-91%	109-117%	107-115%
EDTA plasma(n=5)	89-97%	80-90%	93-102%	102-112%
heparin plasma(n=5)	92-102%	97-108%	95-106%	82-90%

General Annotation

Sub Unit:

Homotetramer; disulfide-linked.

Function:

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Subcellular Location:

Secreted

Database link

UniGene:

Rn.225884

STRING:

10116.ENSRNOP00000019346

KEGG:

rno:100911545

Pfam:

PF00207

Uniprot:

P06238

Related Product

Sequence length:

1472

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1472:

YEKDEFAVAK | YSAPCSTDYG | NA

3D Structure:

Predicted Epitope:

Valid Sequence:

This product has not yet been referenced specifically in any publications.

[1].

"Human alpha2-macroglobulin: genotype-phenotype relation."

(PMID: 14637088)

[2].

"Alpha-2 macroglobulin is genetically associated with Alzheimer disease."

(PMID: 9697696)

[3].

"Cloning of the human alpha 2-macroglobulin gene and detection of mutations in two functional domains: the bait region and the thiolester site."

(PMID: 1370808)

[4].

"Structure of the human alpha-2 macroglobulin gene and its promotor."

(PMID: 1374237)

[5].

"Human alpha 2-macroglobulin gene is located on chromosome 12."

(PMID: 2408344)

[6].

"Association between haplotype -88G/25G in A2M with Alzheimer's disease."

(PMID: 20493925)

[7].

"Interferon gamma receptor 2 gene variants are associated with liver fibrosis in patients with chronic hepatitis C infection."

(PMID: 20587546)

[8].

"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

(PMID: 20628086)

[9].

"Lack of interaction between LRP1 and A2M polymorphisms for the risk of Alzheimer disease."

(PMID: 20637261)

[10].

"Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study."

(PMID: 20800603)

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