A2m (GeneName), Alpha-2-macroglobulin (ProteinName), A2MG_RAT.
Product Name:
Rat A2m/ Alpha-2-macroglobulin ELISA Kit
Cat.#:
E1017r
Brand:
EIAab®
Regulatory Status:
Alternative:
Alpha-2-M, A2m1
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
7.8-500ng/ml
Sensitivity:
4.02ng/mL
Specificity:
Natural and recombinant rat Alpha-2-macroglobulin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:

Research Area:
-
- Data
- Citations
- Publication
- Sequence / 3D
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.2%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤9.8%
Recovery
Recovery was determined by spiking various levels of Alpha-2-macroglobulin into serum and plasma.
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
97 |
91-103 |
Plasma |
99 |
93-105 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-2-macroglobulin and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
98-109% |
81-91% |
109-117% |
107-115% |
EDTA plasma(n=5) |
89-97% |
80-90% |
93-102% |
102-112% |
heparin plasma(n=5) |
92-102%
|
97-108% |
95-106% |
82-90% |
General Annotation
Sub Unit:
Homotetramer; disulfide-linked.
Function:
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Subcellular Location:
Secreted
Database link
UniGene:
STRING:
KEGG:
Pfam:
Uniprot:
This product has not yet been referenced specifically in any publications.
[3].
"Cloning of the human alpha 2-macroglobulin gene and detection of mutations in two functional domains: the bait region and the thiolester site."
[7].
"Interferon gamma receptor 2 gene variants are associated with liver fibrosis in patients with chronic hepatitis C infection."
[8].
"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."
[9].
"Lack of interaction between LRP1 and A2M polymorphisms for the risk of Alzheimer disease."
[10].
"Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study."
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