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A2M (GeneName), Alpha-2-macroglobulin (ProteinName), A2MG_HUMAN.
Product Name:

Human A2M/ Alpha-2-macroglobulin ELISA Kit

Cat.#:

E1017h

Brand:
EIAab®
Regulatory Status:
Alternative:

Alpha-2-M, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, CPAMD5, FWP007

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
7.8-500ng/mL
Sensitivity:
3.9ng/mL
Specificity:
Natural and recombinant human Alpha-2-macroglobulin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human A2M ELISA Kit
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Human A2M ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.6%

Recovery
Recovery was determined by spiking various levels of Alpha-2-macroglobulin into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

94

88-100

Plasma

96

90-102

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-2-macroglobulin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

106-115%

102-112%

104-112%

109-120%

EDTA plasma(n=5)

93-103%

107-117%

100-110%

106-115%

heparin plasma(n=5)

91-100%

 

93-104%

103-115%

112-120%

 

General Annotation


Sub Unit:
Homotetramer; disulfide-linked.


Function:
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.


Subcellular Location:
Secreted


This product has not yet been referenced specifically in any publications.

[1].
"Human alpha2-macroglobulin: genotype-phenotype relation."

[2].
"Alpha-2 macroglobulin is genetically associated with Alzheimer disease."

[3].
"Cloning of the human alpha 2-macroglobulin gene and detection of mutations in two functional domains: the bait region and the thiolester site."

[4].
"Structure of the human alpha-2 macroglobulin gene and its promotor."

[5].
"Human alpha 2-macroglobulin gene is located on chromosome 12."

[6].
"Association between haplotype -88G/25G in A2M with Alzheimer's disease."

[7].
"Interferon gamma receptor 2 gene variants are associated with liver fibrosis in patients with chronic hepatitis C infection."

[8].
"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

[9].
"Lack of interaction between LRP1 and A2M polymorphisms for the risk of Alzheimer disease."

[10].
"Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study."
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