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LRG1 (GeneName), Leucine-rich alpha-2-glycoprotein (ProteinName), A2GL_HUMAN.
Product Name:

Human LRG1/ Leucine-rich alpha-2-glycoprotein ELISA Kit

Cat.#:

E1934h

Brand:
EIAab®
Regulatory Status:
Alternative:

LRG, LRG

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.39ng/mL
Specificity:
Natural and recombinant human Leucine-rich alpha-2-glycoprotein
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human LRG1 ELISA Kit
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Human LRG1 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.2%

Recovery
Recovery was determined by spiking various levels of Leucine-rich alpha-2-glycoprotein into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

102

96-108

Plasma

104

98-110

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Leucine-rich alpha-2-glycoprotein and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

100-109%

91-100%

105-117%

100-112%

EDTA plasma(n=5)

100-112%

111-120%

102-113%

104-116%

heparin plasma(n=5)

105-117%

 

86-96%

87-97%

106-114%

 

General Annotation


Sub Unit:
N/A


Function:
N/A


Subcellular Location:
Secreted


This product has not yet been referenced specifically in any publications.

[1].
"Molecular characterization and expression analysis of leucine-rich alpha2-glycoprotein, a novel marker of granulocytic differentiation."

[2].
"Periodicity of leucine and tandem repetition of a 24-amino acid segment in the primary structure of leucine-rich alpha 2-glycoprotein of human serum."

[3].
"Cytochrome c-induced lymphocyte death from the outside in: inhibition by serum leucine-rich alpha-2-glycoprotein-1."

[4].
"Specific isoforms of leucine-rich alpha2-glycoprotein detected in the proliferative endometrium of women undergoing assisted reproduction are associated with spontaneous pregnancy."

[5].
"Expression of TGF-betas and TGF-beta type II receptor in cerebrospinal fluid of patients with idiopathic normal pressure hydrocephalus."

[6].
"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."

[7].
"Serum leucine-rich alpha-2-glycoprotein-1 binds cytochrome c and inhibits antibody detection of this apoptotic marker in enzyme-linked immunosorbent assay."

[8].
"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."

[9].
"Screening for N-glycosylated proteins by liquid chromatography mass spectrometry."

[10].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
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