LRG1 (GeneName), Leucine-rich alpha-2-glycoprotein (ProteinName), A2GL_HUMAN.
Product Name:
Human LRG1/ Leucine-rich alpha-2-glycoprotein ELISA Kit
Cat.#:
E1934h
Brand:
EIAab®
Regulatory Status:
Alternative:
LRG, LRG
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.39ng/mL
Specificity:
Natural and recombinant human Leucine-rich alpha-2-glycoprotein
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
- Data
- Citations
- Publication
- Sequence / 3D
- Feedback Wall (0)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.1%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤10.2%
Recovery
Recovery was determined by spiking various levels of Leucine-rich alpha-2-glycoprotein into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
102 |
96-108 |
|
Plasma |
104 |
98-110 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Leucine-rich alpha-2-glycoprotein and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
100-109% |
91-100% |
105-117% |
100-112% |
|
EDTA plasma(n=5) |
100-112% |
111-120% |
102-113% |
104-116% |
|
heparin plasma(n=5) |
105-117%
|
86-96% |
87-97% |
106-114% |
General Annotation
Sub Unit:
N/A
Function:
N/A
Subcellular Location:
Secreted
Database link
UniGene:
SMR:
STRING:
KEGG:
MIM:
Pfam:
Uniprot:
Valid Sequence:
This product has not yet been referenced specifically in any publications.
[1].
"Molecular characterization and expression analysis of leucine-rich alpha2-glycoprotein, a novel marker of granulocytic differentiation."
[2].
"Periodicity of leucine and tandem repetition of a 24-amino acid segment in the primary structure of leucine-rich alpha 2-glycoprotein of human serum."
[3].
"Cytochrome c-induced lymphocyte death from the outside in: inhibition by serum leucine-rich alpha-2-glycoprotein-1."
[4].
"Specific isoforms of leucine-rich alpha2-glycoprotein detected in the proliferative endometrium of women undergoing assisted reproduction are associated with spontaneous pregnancy."
[5].
"Expression of TGF-betas and TGF-beta type II receptor in cerebrospinal fluid of patients with idiopathic normal pressure hydrocephalus."
[6].
"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
[7].
"Serum leucine-rich alpha-2-glycoprotein-1 binds cytochrome c and inhibits antibody detection of this apoptotic marker in enzyme-linked immunosorbent assay."
[8].
"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
[9].
"Screening for N-glycosylated proteins by liquid chromatography mass spectrometry."
[10].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
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