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A1cf (基因名), APOBEC1 complementation factor (蛋白名), A1CF_RAT.

产品名称:

Rat A1cf/ APOBEC1 complementation factor ELISA Kit

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

APOBEC1-stimulating protein, Acf, Asp

检测方法:

ELISA

特异性:

Natural and recombinant rat APOBEC1 complementation factor

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

规格 & 价格: 登录.

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产品说明书

数据
文献
出版物
序列 / 3D
常见问题解答
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通用注释

亚单元:

Part of the apolipoprotein B mRNA editing complex with APOBEC1. Found in a complex with APOBEC1 and CELF2/CUGBP2. Interacts APOBEC1. Interacts with TNPO2; TNPO2 may be responsible for transport of A1CF into the nucleus. Interacts with SYNCRIP. Interacts with CELF2/CUGBP2.

功能:

Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also seems to protect the edited APOB mRNA from nonsense-mediated decay.

亚细胞位置:

Nucleus Endoplasmic reticulum Cytoplasm Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum. Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1.

数据库链接

UniGene:

Rn.162520

SMR:

Q923K9

STRING:

10116.ENSRNOP00000062802

KEGG:

rno:170912

Pfam:

PF00076

Uniprot:

Q923K9

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序列长度:

594

序列:

50:

100:

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550:

594:

ATAPVSTAQL | KQAVTLGQDL | AAYTTYEVYP | TFAVTTRGDG | YGTF

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"The apolipoprotein B mRNA editing complex performs a multifunctional cycle and suppresses nonsense-mediated decay."

(PMID: 12881431)

[2].

"A novel nuclear localization signal in the auxiliary domain of apobec-1 complementation factor regulates nucleocytoplasmic import and shuttling."

(PMID: 12896982)

[3].

"Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing."

(PMID: 11815617)

[4].

"Identification of domains in apobec-1 complementation factor required for RNA binding and apolipoprotein-B mRNA editing."

(PMID: 11871661)

[5].

"Isolation, characterization and developmental regulation of the human apobec-1 complementation factor (ACF) gene."

(PMID: 11718896)

[6].

"Molecular cloning of apobec-1 complementation factor, a novel RNA-binding protein involved in the editing of apolipoprotein B mRNA."

(PMID: 10669759)

[7].

"Targeted deletion of the murine apobec-1 complementation factor (acf) gene results in embryonic lethality."

(PMID: 16055734)

[8].

"Complete sequencing and characterization of 21,243 full-length human cDNAs."

(PMID: 14702039)

[9].

"The DNA sequence and comparative analysis of human chromosome 10."

(PMID: 15164054)

[10].

"Identification of GRY-RBP as an apolipoprotein B RNA-binding protein that interacts with both apobec-1 and apobec-1 complementation factor to modulate C to U editing."

(PMID: 11134005)

[1].

大鼠A1cfELISA试剂盒可以做多少个样本？

大鼠A1cfELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

大鼠A1cfELISA试剂盒使用视频？

大鼠A1cfELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

大鼠A1cfELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的大鼠A1cfELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

大鼠A1cfELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

大鼠A1cfELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析大鼠A1cfELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

大鼠A1cfELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集大鼠A1cfELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

大鼠A1cfELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

大鼠A1cfELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

大鼠A1cfELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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