Home > ELISA Kit > Rat Serpina1 ELISA Kit

Serpina1 (GeneName), Alpha-1-antiproteinase (ProteinName), A1AT_RAT.

Product Name:

Rat Serpina1/ Alpha-1-antiproteinase ELISA Kit

Cat.#:

E1697r

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

Alpha-1-antitrypsin, Alpha-1-proteinase inhibitor, Serpin A1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/ml

Sensitivity:

0.809ng/mL

Specificity:

Natural and recombinant rat Alpha-1-antiproteinase

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

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Rat Serpina1 ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations
Publication
Sequence / 3D
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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.1%

Recovery

Recovery was determined by spiking various levels of Alpha-1-antiproteinase into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	102	96-108
Plasma	104	98-110

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-1-antiproteinase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	108-118%	90-99%	93-104%	100-111%
EDTA plasma(n=5)	105-117%	104-116%	108-117%	89-99%
heparin plasma(n=5)	110-120%	106-115%	82-92%	103-113%

General Annotation

Sub Unit:

N/A

Function:

Inhibitor of serine proteases. The primary target is elastase, but also has a moderate affinity for plasmin and thrombin.

Subcellular Location:

Secreted

Database link

UniGene:

Rn.1419

SMR:

P17475

STRING:

10116.ENSRNOP00000012577

KEGG:

rno:24648

Pfam:

PF00079

Uniprot:

P17475

Related Product

Human SERPINA1 ELISA Kit

Bovine SERPINA1 ELISA Kit

Pig SERPINA1 ELISA Kit

Human SERPINA1 CLIA Kit

Bovine SERPINA1 CLIA Kit

Sequence length:

411

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

411:

LFVGKVIDPT | R

3D Structure:

Predicted Epitope:

Valid Sequence:

This product has not yet been referenced specifically in any publications.

[1].

"Polymorphisms in the SERPINA1 (Alpha-1-Antitrypsin) gene are associated with severe chronic rhinosinusitis unresponsive to medical therapy."

(PMID: 20109307)

[2].

"Prevalence of genetic polymorphisms in the promoter region of the alpha-1 antitrypsin (SERPINA1) gene in chronic liver disease: a case control study."

(PMID: 20170533)

[3].

"Alpha-1-antitrypsin phenotypes in adult liver disease patients."

(PMID: 19961268)

[4].

"Real time PCR detection of the PI*Z and PI*S mutations associated with alpha-1 antitrypsin deficiency."

(PMID: 19956452)

[5].

"Prevalence of common HFE and SERPINA1 mutations in patients with hepatocellular carcinoma in a Moroccan population."

(PMID: 18164971)

[6].

"SERPINA1 gene variants in individuals from the general population with reduced alpha1-antitrypsin concentrations."

(PMID: 18515255)

[7].

"Isoelectric focusing phenotyping and denaturing gradient gel electrophoresis genotyping: a comparison of two methods in detection of alpha-1-antitrypsin variants."

(PMID: 18433707)

[8].

"Determination of alpha 1-antitrypsin levels and of the presence of S and Z alleles in a population of patients with chronic respiratory symptoms."

(PMID: 19180336)

[9].

"An alpha-1 antitrypsin genetic variant from India."

(PMID: 17650587)

[10].

"Haplotypes of the alpha-1 antitrypsin gene in healthy controls and Z deficiency patients."

(PMID: 15532029)

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