Serpina1 (GeneName), Alpha-1-antiproteinase (ProteinName), A1AT_RAT.
Product Name:
Rat Serpina1/ Alpha-1-antiproteinase ELISA Kit
Cat.#:
E1697r
Brand:
EIAab®
Regulatory Status:
Alternative:
Alpha-1-antitrypsin, Alpha-1-proteinase inhibitor, Serpin A1
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100ng/ml
Sensitivity:
0.809ng/mL
Specificity:
Natural and recombinant rat Alpha-1-antiproteinase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:

Research Area:
-
- Data
- Citations
- Publication
- Sequence / 3D
- Feedback Wall (0)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.5%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤9.1%
Recovery
Recovery was determined by spiking various levels of Alpha-1-antiproteinase into serum and plasma.
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
102 |
96-108 |
Plasma |
104 |
98-110 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-1-antiproteinase and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
108-118% |
90-99% |
93-104% |
100-111% |
EDTA plasma(n=5) |
105-117% |
104-116% |
108-117% |
89-99% |
heparin plasma(n=5) |
110-120%
|
106-115% |
82-92% |
103-113% |
General Annotation
Sub Unit:
N/A
Function:
Inhibitor of serine proteases. The primary target is elastase, but also has a moderate affinity for plasmin and thrombin.
Subcellular Location:
Secreted
This product has not yet been referenced specifically in any publications.
[1].
"Polymorphisms in the SERPINA1 (Alpha-1-Antitrypsin) gene are associated with severe chronic rhinosinusitis unresponsive to medical therapy."
[2].
"Prevalence of genetic polymorphisms in the promoter region of the alpha-1 antitrypsin (SERPINA1) gene in chronic liver disease: a case control study."
[4].
"Real time PCR detection of the PI*Z and PI*S mutations associated with alpha-1 antitrypsin deficiency."
[5].
"Prevalence of common HFE and SERPINA1 mutations in patients with hepatocellular carcinoma in a Moroccan population."
[6].
"SERPINA1 gene variants in individuals from the general population with reduced alpha1-antitrypsin concentrations."
[7].
"Isoelectric focusing phenotyping and denaturing gradient gel electrophoresis genotyping: a comparison of two methods in detection of alpha-1-antitrypsin variants."
[8].
"Determination of alpha 1-antitrypsin levels and of the presence of S and Z alleles in a population of patients with chronic respiratory symptoms."
[10].
"Haplotypes of the alpha-1 antitrypsin gene in healthy controls and Z deficiency patients."
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