SERPINA1 (GeneName), Alpha-1-antitrypsin (ProteinName), A1AT_HUMAN.
Product Name:
Human SERPINA1/ Alpha-1-antitrypsin ELISA Kit
Cat.#:
E1697h
Brand:
EIAab®
Regulatory Status:
Alternative:
Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1, AAT, PI, PRO0684, PRO2209
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
3.12-200ng/mL
Sensitivity:
1.2ng/mL
Specificity:
Natural and recombinant human Alpha-1-antitrypsin
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Development Biology
- Data
- Citations
- Publication
- Sequence / 3D
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.7%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤5.6%
Recovery
Recovery was determined by spiking various levels of Alpha-1-antitrypsin into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
99 |
93-105 |
|
Plasma |
101 |
95-107 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-1-antitrypsin and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
86-98% |
105-114% |
90-100% |
100-112% |
|
EDTA plasma(n=5) |
101-114% |
105-116% |
89-98% |
90-99% |
|
heparin plasma(n=5) |
100-112%
|
108-118% |
97-108% |
105-115% |
General Annotation
Sub Unit:
The variants S and Z interact with CANX AND PDIA3.
Function:
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Subcellular Location:
Short peptide from AAT
Secreted
Extracellular space
Extracellular matrix
Database link
Valid Sequence:
This product has not yet been referenced specifically in any publications.
[1].
"Polymorphisms in the SERPINA1 (Alpha-1-Antitrypsin) gene are associated with severe chronic rhinosinusitis unresponsive to medical therapy."
[2].
"Prevalence of genetic polymorphisms in the promoter region of the alpha-1 antitrypsin (SERPINA1) gene in chronic liver disease: a case control study."
[4].
"Real time PCR detection of the PI*Z and PI*S mutations associated with alpha-1 antitrypsin deficiency."
[5].
"Prevalence of common HFE and SERPINA1 mutations in patients with hepatocellular carcinoma in a Moroccan population."
[6].
"SERPINA1 gene variants in individuals from the general population with reduced alpha1-antitrypsin concentrations."
[7].
"Isoelectric focusing phenotyping and denaturing gradient gel electrophoresis genotyping: a comparison of two methods in detection of alpha-1-antitrypsin variants."
[8].
"Determination of alpha 1-antitrypsin levels and of the presence of S and Z alleles in a population of patients with chronic respiratory symptoms."
[10].
"Haplotypes of the alpha-1 antitrypsin gene in healthy controls and Z deficiency patients."
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