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Home  >  ELISA Kit  >  Human ORM2 ELISA Kit
ORM2 (GeneName), Alpha-1-acid glycoprotein 2 (ProteinName), A1AG2_HUMAN.
Product Name:

Human ORM2/ Alpha-1-acid glycoprotein 2 ELISA Kit

Cat.#:

E1780h

Brand:
EIAab®
Regulatory Status:
Alternative:

AGP 2, Orosomucoid-2, OMD 2, AGP2

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.625-40ng/mL
Sensitivity:
0.12ng/mL
Specificity:
Natural and recombinant human Alpha-1-acid glycoprotein 2
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human ORM2 ELISA Kit
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Human ORM2 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.4%

Recovery
Recovery was determined by spiking various levels of Alpha-1-acid glycoprotein 2 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

104

98-110

Plasma

106

100-112

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-1-acid glycoprotein 2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

83-93%

98-108%

105-116%

91-100%

EDTA plasma(n=5)

84-94%

104-115%

90-100%

88-97%

heparin plasma(n=5)

93-104%

 

110-120%

84-96%

104-113%

 

General Annotation


Sub Unit:
N/A


Function:
Functions as transport protein in the blood stream. Binds various hydrophobic ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability. Appears to function in modulating the activity of the immune system during the acute-phase reaction.


Subcellular Location:
Secreted


This product has not yet been referenced specifically in any publications.

[1].
"Genetic determinants of warfarin dosing in the Han-Chinese population."

[2].
"Structure and characterisation of a duplicated human alpha 1 acid glycoprotein gene."

[3].
"Investigation of genetic variants of alpha-1 acid glycoprotein by ultra-performance liquid chromatography-mass spectrometry."

[4].
"Clinical relevance of a pharmacogenetic approach using multiple candidate genes to predict response and resistance to imatinib therapy in chronic myeloid leukemia."

[5].
"Association of warfarin dose with genes involved in its action and metabolism."

[6].
"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."

[7].
"Screening for N-glycosylated proteins by liquid chromatography mass spectrometry."

[8].
"A proteomic analysis of human bile."

[9].
"A new strategy for identification of N-glycosylated proteins and unambiguous assignment of their glycosylation sites using HILIC enrichment and partial deglycosylation."

[10].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
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