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Orm1 (GeneName), Alpha-1-acid glycoprotein 1 (ProteinName), A1AG1_MOUSE.
Product Name:

Mouse Orm1/ Alpha-1-acid glycoprotein 1 ELISA Kit

Cat.#:

E0816m

Brand:
EIAab®
Regulatory Status:
Alternative:

AGP 1, Orosomucoid-1, OMD 1, Agp1, Orm-1

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100ng/mL
Sensitivity:
0.89ng/mL
Specificity:
Natural and recombinant mouse Alpha-1-acid glycoprotein 1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Immunology
Mouse Orm1 ELISA Kit
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Mouse Orm1 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.0%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.9%

Recovery
Recovery was determined by spiking various levels of Alpha-1-acid glycoprotein 1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

97

91-103

Plasma

99

93-105

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-1-acid glycoprotein 1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

97-106%

104-113%

104-114%

98-109%

EDTA plasma(n=5)

95-105%

90-101%

92-103%

103-113%

heparin plasma(n=5)

83-83%

 

101-110%

87-96%

102-112%

 

General Annotation


Sub Unit:
N/A


Function:
Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain (By similarity). Appears to function in modulating the activity of the immune system during the acute-phase reaction.


Subcellular Location:
Secreted


This product has not yet been referenced specifically in any publications.

[1].
"Orosomucoid influences the response to antidepressants in major depressive disorder."

[2].
"Human orosomucoid polymorphism: molecular basis of the three common ORM1 alleles, ORM1*F1, ORM1*F2, and ORM1*S."

[3].
"Genetic factors (VKORC1, CYP2C9, EPHX1, and CYP4F2) are predictor variables for warfarin response in very elderly, frail inpatients."

[4].
"The IFNG (IFN-gamma) genotype predicts cytogenetic and molecular response to imatinib therapy in chronic myeloid leukemia."

[5].
"Investigation of genetic variants of alpha-1 acid glycoprotein by ultra-performance liquid chromatography-mass spectrometry."

[6].
"Genetic variants in the candidate genes of the apoptosis pathway and susceptibility to chronic myeloid leukemia."

[7].
"Genetic determinants of warfarin dosing in the Han-Chinese population."

[8].
"Genetic factors contribute to patient-specific warfarin dose for Han Chinese."

[9].
"Population pharmacokinetics and pharmacogenetics of imatinib in children and adults."

[10].
"The 1.8-A crystal structure of alpha1-acid glycoprotein (Orosomucoid) solved by UV RIP reveals the broad drug-binding activity of this human plasma lipocalin."
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