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Atg16l1 (基因名), Autophagy-related protein 16-1 (蛋白名), A16L1_MOUSE.

产品名称:

Mouse Atg16l1/ Autophagy-related protein 16-1 ELISA Kit

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

APG16-like 1, Apg16l

检测方法:

ELISA

特异性:

Natural and recombinant mouse Autophagy-related protein 16-1

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

Neurosciences

规格 & 价格: 登录.

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Mouse Atg16l1 ELISA Kit

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产品说明书

数据
文献
出版物
序列 / 3D
常见问题解答
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通用注释

亚单元:

Homodimer (By similarity). Homooligomer (PubMed:12665549). Interacts with WIPI2 (PubMed:24954904). Interacts with RB1CC1; the interaction is required for ULK1 complex-dependent autophagy (PubMed:23392225, PubMed:23262492). Interacts with ATG5 (PubMed:12665549). Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Interacts with RAB33B (PubMed:18448665). Interacts (via WD repeats) with TMEM59; the interaction mediates unconventional autophagic activity of TMEM59 (By similarity). Interacts with TLR2 and NOD2 (By similarity). Interacts (via WD repeats) with MEFV (By similarity). Interacts (via N-terminal) with CLTC.

功能:

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:18849966, PubMed:12665549, PubMed:24954904, PubMed:24553140, PubMed:23392225). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (By similarity). Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed:24238340). Plays a role in regulating morphology and function of Paneth cell (PubMed:18849966).

亚细胞位置:

Cytoplasm Preautophagosomal structure membrane Peripheral membrane protein Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme.

数据库链接

UniGene:

Mm.272972

SMR:

Q8C0J2

STRING:

10090.ENSMUSP00000108815

KEGG:

mmu:77040

Pfam:

PF08614

Uniprot:

Q8C0J2

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序列长度:

607

序列:

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AVLWAQP

3D 模型:

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免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Autophagy 16-like 1 rs2241880 G allele is associated with Crohn's disease in German children."

(PMID: 19659808)

[2].

"ATG16L1 and IL23R are associated with inflammatory bowel diseases but not with celiac disease in the Netherlands."

(PMID: 18047540)

[3].

"A genome-wide association scan of nonsynonymous SNPs identifies a susceptibility variant for Crohn disease in ATG16L1."

(PMID: 17200669)

[4].

"Replication and meta-analysis of 13,000 cases defines the risk for interleukin-23 receptor and autophagy-related 16-like 1 variants in Crohn's disease."

(PMID: 20485703)

[5].

"NOD2/CARD15, ATG16L1 and IL23R gene polymorphisms and childhood-onset of Crohn's disease."

(PMID: 20380008)

[6].

"Interaction of the major inflammatory bowel disease susceptibility alleles in Crohn's disease patients."

(PMID: 20066736)

[7].

"Association of IL23R p.381Gln and ATG16L1 p.197Ala with Crohn disease in the Czech population."

(PMID: 19590455)

[8].

"Autophagy gene ATG16L1 but not IRGM is associated with Crohn's disease in Canadian children."

(PMID: 18985712)

[9].

"Molecular prediction of disease risk and severity in a large Dutch Crohn's disease cohort."

(PMID: 18824555)

[10].

"A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells."

(PMID: 18849966)

[1].

小鼠Atg16l1ELISA试剂盒可以做多少个样本？

小鼠Atg16l1ELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠Atg16l1ELISA试剂盒使用视频？

小鼠Atg16l1ELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠Atg16l1ELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠Atg16l1ELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠Atg16l1ELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠Atg16l1ELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠Atg16l1ELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠Atg16l1ELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠Atg16l1ELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠Atg16l1ELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠Atg16l1ELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠Atg16l1ELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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