ALDH16A1 (GeneName), Aldehyde dehydrogenase family 16 member A1 (ProteinName), A16A1_HUMAN.
Product Name:
Human ALDH16A1/ Aldehyde dehydrogenase family 16 member A1 ELISA Kit
Cat.#:
E11327h
Brand:
EIAab®
Regulatory Status:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.31ng/ml
Specificity:
Natural and recombinant human Aldehyde dehydrogenase family 16 member A1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
- Data
- Citations
- Publication
- Sequence / 3D
- Feedback Wall (0)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤9.2%
Recovery
Recovery was determined by spiking various levels of Aldehyde dehydrogenase family 16 member A1 into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
96 |
90-102 |
|
Plasma |
98 |
92-104 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aldehyde dehydrogenase family 16 member A1 and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
88-98% |
88-98% |
107-116% |
90-102% |
|
EDTA plasma(n=5) |
90-90% |
98-108% |
92-102% |
107-117% |
|
heparin plasma(n=5) |
86-98%
|
98-108% |
89-99% |
99-109% |
General Annotation
Sub Unit:
Interacts with SPG21.
Function:
N/A
Subcellular Location:
N/A
Database link
UniGene:
SMR:
STRING:
KEGG:
MIM:
Pfam:
Uniprot:
Valid Sequence:
This product has not yet been referenced specifically in any publications.
[1].
"Interaction of the SPG21 protein ACP33/maspardin with the aldehyde dehydrogenase ALDH16A1."
[3].
"Architecture of the human interactome defines protein communities and disease networks."
[4].
"Temporal proteomics of NGF-TrkA signaling identifies an inhibitory role for the E3 ligase Cbl-b in neuroblastoma cell differentiation."
[7].
"A human interactome in three quantitative dimensions organized by stoichiometries and abundances."
[9].
"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
[10].
"ALDH16A1 is a novel non-catalytic enzyme that may be involved in the etiology of gout via protein-protein interactions with HPRT1."
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