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Nudt1 (GeneName), 7,8-dihydro-8-oxoguanine triphosphatase (ProteinName), 8ODP_MOUSE.
Product Name:

Mouse Nudt1/ 7,8-dihydro-8-oxoguanine triphosphatase ELISA Kit

Cat.#:

E3104m

Brand:
EIAab®
Regulatory Status:
Alternative:

2-hydroxy-dATP diphosphatase, 8-oxo-dGTPase, Nucleoside diphosphate-linked moiety X motif 1, Nudix motif 1, Mth1

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.413ng/mL
Specificity:
Natural and recombinant mouse 7,8-dihydro-8-oxoguanine triphosphatase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Mouse Nudt1 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.5%

Recovery
Recovery was determined by spiking various levels of 7,8-dihydro-8-oxoguanine triphosphatase into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

87

81-93

Plasma

89

83-95

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 7,8-dihydro-8-oxoguanine triphosphatase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

92-104%

94-106%

98-110%

105-114%

EDTA plasma(n=5)

99-109%

99-109%

106-116%

100-110%

heparin plasma(n=5)

100-108%

 

96-106%

85-96%

100-110%

 

General Annotation


Sub Unit:
Monomer.


Function:
Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.


Subcellular Location:
Cytoplasm Nucleus Nucleus membrane Cytoplasmic vesicle Secretory vesicle Acrosome


This product has not yet been referenced specifically in any publications.

[1].
"Association of polymorphisms in the MTH1 gene with small cell lung carcinoma risk."

[2].
"Association study of human MTH1 gene polymorphisms with type 1 diabetes mellitus."

[3].
"Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis."

[4].
"Association study of human MTH1 Ile45Thr polymorphism with sporadic Parkinson's disease."

[5].
"Analysis of the base excision repair genes MTH1, OGG1 and MUTYH in patients with squamous oral carcinomas."

[6].
"MYH, OGG1, MTH1, and APC alterations involved in the colorectal tumorigenesis of Korean patients with multiple adenomas."

[7].
"Genetic investigation of DNA-repair pathway genes PMS2, MLH1, MSH2, MSH6, MUTYH, OGG1 and MTH1 in sporadic colon cancer."

[8].
"Heterogeneous molecular mechanisms underlie attenuated familial adenomatous polyposis."

[9].
"The GT to GC single nucleotide polymorphism at the beginning of an alternative exon 2C of human MTH1 gene confers an amino terminal extension that functions as a mitochondrial targeting signal."

[10].
"Germline susceptibility to colorectal cancer due to base-excision repair gene defects."
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