NUDT1 (GeneName), 7,8-dihydro-8-oxoguanine triphosphatase (ProteinName), 8ODP_HUMAN.
Product Name:
Human NUDT1/ 7,8-dihydro-8-oxoguanine triphosphatase ELISA Kit
Cat.#:
E3104h
Brand:
EIAab®
Regulatory Status:
Alternative:
2-hydroxy-dATP diphosphatase, 8-oxo-dGTPase, Nucleoside diphosphate-linked moiety X motif 1, Nudix motif 1, MTH1
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.23ng/mL
Specificity:
Natural and recombinant human 7,8-dihydro-8-oxoguanine triphosphatase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
-
- Data
- Citations
- Publication
- Sequence / 3D
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤6.1%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤8.5%
Recovery
Recovery was determined by spiking various levels of 7,8-dihydro-8-oxoguanine triphosphatase into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
86 |
80-92 |
|
Plasma |
88 |
82-94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 7,8-dihydro-8-oxoguanine triphosphatase and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
100-113% |
98-108% |
95-104% |
81-91% |
|
EDTA plasma(n=5) |
80-89% |
111-123% |
91-101% |
84-93% |
|
heparin plasma(n=5) |
103-112%
|
102-112% |
92-102% |
95-105% |
General Annotation
Sub Unit:
Monomer.
Function:
Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.
Subcellular Location:
Isoform p26
Cytoplasm
Mitochondrion matrix
Database link
Valid Sequence:
This product has not yet been referenced specifically in any publications.
[1].
"Association of polymorphisms in the MTH1 gene with small cell lung carcinoma risk."
[2].
"Association study of human MTH1 gene polymorphisms with type 1 diabetes mellitus."
[3].
"Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis."
[4].
"Association study of human MTH1 Ile45Thr polymorphism with sporadic Parkinson's disease."
[5].
"Analysis of the base excision repair genes MTH1, OGG1 and MUTYH in patients with squamous oral carcinomas."
[6].
"MYH, OGG1, MTH1, and APC alterations involved in the colorectal tumorigenesis of Korean patients with multiple adenomas."
[7].
"Genetic investigation of DNA-repair pathway genes PMS2, MLH1, MSH2, MSH6, MUTYH, OGG1 and MTH1 in sporadic colon cancer."
[8].
"Heterogeneous molecular mechanisms underlie attenuated familial adenomatous polyposis."
[9].
"The GT to GC single nucleotide polymorphism at the beginning of an alternative exon 2C of human MTH1 gene confers an amino terminal extension that functions as a mitochondrial targeting signal."
[10].
"Germline susceptibility to colorectal cancer due to base-excision repair gene defects."
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