Home > ELISA Kit > Rat Scg5 ELISA Kit

Scg5 (GeneName), Neuroendocrine protein 7B2 (ProteinName), 7B2_RAT.

Product Name:

Rat Scg5/ Neuroendocrine protein 7B2 ELISA Kit

Cat.#:

E9861r

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

Secretogranin V, Secretogranin-5, Secretory granule endocrine protein I, Sgne1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

78-5000pg/mL

Sensitivity:

43pg/mL

Specificity:

Natural and recombinant rat Neuroendocrine protein 7B2

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Neurosciences

Size & Price: Sign In.

Attention

Rat Scg5 ELISA Kit

Email *

Message *

Please Sign In.

Product Datasheets

Instruction:

MSDS:

Data
Citations
Sequence / 3D
Feedback Wall (0)

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.8%

Recovery

Recovery was determined by spiking various levels of Neuroendocrine protein 7B2 into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	96	90-102
Plasma	98	92-104

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neuroendocrine protein 7B2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	103-113%	96-106%	107-117%	110-120%
EDTA plasma(n=5)	108-118%	113-125%	99-109%	91-101%
heparin plasma(n=5)	92-101%	106-116%	102-113%	93-103%

General Annotation

Sub Unit:

Interacts with PCSK2/PC2 early in the secretory pathway. Dissociation occurs at later stages.

Function:

Acts as a molecular chaperone for PCSK2/PC2, preventing its premature activation in the regulated secretory pathway. Binds to inactive PCSK2 in the endoplasmic reticulum and facilitates its transport from there to later compartments of the secretory pathway where it is proteolytically matured and activated. Also required for cleavage of PCSK2 but does not appear to be involved in its folding. Plays a role in regulating pituitary hormone secretion. The C-terminal peptide inhibits PCSK2 in vitro.

Subcellular Location:

Secreted Neuroendocrine and endocrine secretory granules.

Database link

UniGene:

Rn.6173

STRING:

10116.ENSRNOP00000010679

KEGG:

rno:25719

Pfam:

PF05281

Uniprot: