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SCG5 (GeneName), Neuroendocrine protein 7B2 (ProteinName), 7B2_HUMAN.
Product Name:

Human SCG5/ Neuroendocrine protein 7B2 ELISA Kit

Cat.#:

E9861h

Brand:
EIAab®
Regulatory Status:
Alternative:

Pituitary polypeptide, Secretogranin V, Secretogranin-5, Secretory granule endocrine protein I, SGNE1

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
31.2-2000pg/mL
Sensitivity:
16pg/mL
Specificity:
Natural and recombinant human Neuroendocrine protein 7B2
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Neurosciences
Human SCG5 ELISA Kit
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Human SCG5 ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.0%

Recovery
Recovery was determined by spiking various levels of Neuroendocrine protein 7B2 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

89

83-95

Plasma

91

85-97

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neuroendocrine protein 7B2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

107-116%

104-114%

88-98%

100-111%

EDTA plasma(n=5)

115-127%

106-117%

85-96%

106-115%

heparin plasma(n=5)

82-91%

 

105-117%

105-115%

111-121%

 

General Annotation


Sub Unit:
Interacts with PCSK2/PC2 early in the secretory pathway. Dissociation occurs at later stages.


Function:
Acts as a molecular chaperone for PCSK2/PC2, preventing its premature activation in the regulated secretory pathway. Binds to inactive PCSK2 in the endoplasmic reticulum and facilitates its transport from there to later compartments of the secretory pathway where it is proteolytically matured and activated. Also required for cleavage of PCSK2 but does not appear to be involved in its folding. Plays a role in regulating pituitary hormone secretion. The C-terminal peptide inhibits PCSK2 in vitro.


Subcellular Location:
Secreted Neuroendocrine and endocrine secretory granules.


This product has not yet been referenced specifically in any publications.
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