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PGD (GeneName), 6-phosphogluconate dehydrogenase, decarboxylating (ProteinName), 6PGD_HUMAN.
Product Name:

Human PGD/ 6-phosphogluconate dehydrogenase, decarboxylating ELISA Kit

Cat.#:

E2120h

Brand:
EIAab®
Regulatory Status:
Alternative:

PGDH

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100umol/L
Sensitivity:
0.78umol/L
Specificity:
Natural and recombinant human 6-phosphogluconate dehydrogenase, decarboxylating
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Human PGD ELISA Kit
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Human PGD ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.1%

Recovery
Recovery was determined by spiking various levels of 6-phosphogluconate dehydrogenase, decarboxylating into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

93

87-99

Plasma

95

89-101

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 6-phosphogluconate dehydrogenase, decarboxylating and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

99-108%

97-107%

101-111%

85-95%

EDTA plasma(n=5)

102-112%

90-101%

103-114%

114-124%

heparin plasma(n=5)

109-120%

 

88-97%

90-99%

109-119%

 

General Annotation


Sub Unit:
Homodimer.


Function:
Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.


Subcellular Location:
Cytoplasm


This product has not yet been referenced specifically in any publications.

[1].
"Identification of a cDNA encoding 6-phosphogluconate dehydrogenase from a human heart cDNA library."

[2].
"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

[3].
"Genome-wide association study identifies 1p36.22 as a new susceptibility locus for hepatocellular carcinoma in chronic hepatitis B virus carriers."

[4].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

[5].
"6-Phosphogluconate dehydrogenase: the mechanism of action investigated by a comparison of the enzyme from different species."

[6].
"Inhibiting 6-phosphogluconate dehydrogenase reverses doxorubicin resistance in anaplastic thyroid cancer via inhibiting NADPH-dependent metabolic reprogramming."

[7].
"Mapping the Genetic Landscape of Human Cells."

[8].
"Loss of ER retention motif of AGR2 can impact mTORC signaling and promote cancer metastasis."

[9].
"TTF-1/NKX2-1 binds to DDB1 and confers replication stress resistance to lung adenocarcinomas."

[10].
"Blocking an N-terminal acetylation-dependent protein interaction inhibits an E3 ligase."
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