Home > ELISA Kit > Rat Nt5e ELISA Kit

Nt5e (GeneName), 5'-nucleotidase (ProteinName), 5NTD_RAT.

Product Name:

Rat Nt5e/ 5'-nucleotidase ELISA Kit

Cat.#:

E0104r

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

5'-NT, Ecto-5'-nucleotidase, CD73, Nt5, Nte

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.169ng/mL

Specificity:

Natural and recombinant rat 5'-nucleotidase

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Cardiovascular

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Rat Nt5e ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations
Publication
Sequence / 3D
Feedback Wall (0)

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.8%

Recovery

Recovery was determined by spiking various levels of 5'-nucleotidase into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	95	89-101
Plasma	97	91-103

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5'-nucleotidase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	98-107%	92-102%	85-94%	95-104%
EDTA plasma(n=5)	106-116%	100-109%	98-107%	95-107%
heparin plasma(n=5)	116-128%	112-122%	99-108%	88-98%

General Annotation

Sub Unit:

Homodimer.

Function:

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

Subcellular Location:

Cell membrane Lipid-anchor GPI-anchor

Database link

UniGene:

Rn.40132

SMR:

P21588

STRING:

10116.ENSRNOP00000015057

Pfam:

PF02872

Uniprot:

P21588

Related Product

Human NT5E ELISA Kit

Mouse Nt5e ELISA Kit

Bovine NT5E ELISA Kit

Human NT5E CLIA Kit

Mouse Nt5e CLIA Kit

Sequence length:

576

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

576:

SAASHYQGSF | PLIILSFWAV | ILVLYQ

3D Structure:

Predicted Epitope:

Valid Sequence:

This product has not yet been referenced specifically in any publications.

[1].

"Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene."

(PMID: 8566797)

[2].

"NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein."

(PMID: 24887587)

[3].

"The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism."

(PMID: 21933152)

[4].

"Crystal structure of the human ecto-5'-nucleotidase (CD73): insights into the regulation of purinergic signaling."

(PMID: 23142347)

[5].

"NT5E mutations and arterial calcifications."

(PMID: 21288095)

[6].

"Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"."

(PMID: 20628624)

[7].

"[Role of PKC in regulation of CD73 by lysophosphatidylcholine in human endothelial cells]."

(PMID: 20476579)

[8].

"Contribution of adenosine related genes to the risk of depression with disturbed sleep."

(PMID: 20392501)

[9].

"Explorative study to identify novel candidate genes related to oxaliplatin efficacy and toxicity using a DNA repair array."

(PMID: 19536092)

[10].

"Ecto-5'-nucleotidase promotes invasion, migration and adhesion of human breast cancer cells."