Nt5e (GeneName), 5'-nucleotidase (ProteinName), 5NTD_RAT.
Product Name:
Rat Nt5e/ 5'-nucleotidase ELISA Kit
Cat.#:
E0104r
Brand:
EIAab®
Regulatory Status:
Alternative:
5'-NT, Ecto-5'-nucleotidase, CD73, Nt5, Nte
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.169ng/mL
Specificity:
Natural and recombinant rat 5'-nucleotidase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:

Research Area:
Cardiovascular
- Data
- Citations
- Publication
- Sequence / 3D
- Feedback Wall (0)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.7%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤6.8%
Recovery
Recovery was determined by spiking various levels of 5'-nucleotidase into serum and plasma.
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
95 |
89-101 |
Plasma |
97 |
91-103 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5'-nucleotidase and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
98-107% |
92-102% |
85-94% |
95-104% |
EDTA plasma(n=5) |
106-116% |
100-109% |
98-107% |
95-107% |
heparin plasma(n=5) |
116-128%
|
112-122% |
99-108% |
88-98% |
General Annotation
Sub Unit:
Homodimer.
Function:
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Subcellular Location:
Cell membrane
Lipid-anchor
GPI-anchor
Database link
This product has not yet been referenced specifically in any publications.
[1].
"Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene."
[2].
"NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein."
[3].
"The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism."
[4].
"Crystal structure of the human ecto-5'-nucleotidase (CD73): insights into the regulation of purinergic signaling."
[6].
"Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"."
[7].
"[Role of PKC in regulation of CD73 by lysophosphatidylcholine in human endothelial cells]."
[8].
"Contribution of adenosine related genes to the risk of depression with disturbed sleep."
[9].
"Explorative study to identify novel candidate genes related to oxaliplatin efficacy and toxicity using a DNA repair array."
[10].
"Ecto-5'-nucleotidase promotes invasion, migration and adhesion of human breast cancer cells."
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