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Home  >  ELISA Kit  >  Human NT5E ELISA Kit
NT5E (GeneName), 5'-nucleotidase (ProteinName), 5NTD_HUMAN.
Product Name:

Human NT5E/ 5'-nucleotidase ELISA Kit

Cat.#:

E0104h

Brand:
EIAab®
Regulatory Status:
Alternative:

5'-NT, Ecto-5'-nucleotidase, CD73, NT5, NTE

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.183 ng/mL
Specificity:
Natural and recombinant human 5'-nucleotidase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cardiovascular
Human NT5E ELISA Kit
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Human NT5E ELISA Kit
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Product Datasheets
Datasheet: Down Datasheet
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.6%

Recovery
Recovery was determined by spiking various levels of 5'-nucleotidase into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

96

90-102

Plasma

98

92-104

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5'-nucleotidase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

92-101%

90-100%

104-115%

108-119%

EDTA plasma(n=5)

91-102%

86-96%

82-92%

95-107%

heparin plasma(n=5)

106-116%

 

107-117%

106-115%

90-99%

 

General Annotation


Sub Unit:
Homodimer.


Function:
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.


Subcellular Location:
Cell membrane Lipid-anchor GPI-anchor


This product has not yet been referenced specifically in any publications.

[1].
"Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene."

[2].
"NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein."

[3].
"The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism."

[4].
"Crystal structure of the human ecto-5'-nucleotidase (CD73): insights into the regulation of purinergic signaling."

[5].
"NT5E mutations and arterial calcifications."

[6].
"Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility "hot-spot"."

[7].
"[Role of PKC in regulation of CD73 by lysophosphatidylcholine in human endothelial cells]."

[8].
"Contribution of adenosine related genes to the risk of depression with disturbed sleep."

[9].
"Explorative study to identify novel candidate genes related to oxaliplatin efficacy and toxicity using a DNA repair array."

[10].
"Ecto-5'-nucleotidase promotes invasion, migration and adhesion of human breast cancer cells."
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