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Nt5c3l (GeneName), Cytosolic 5'-nucleotidase III-like protein (ProteinName), 5NT3L_RAT.
Product Name:

Rat Nt5c3l/ Cytosolic 5'-nucleotidase III-like protein ELISA Kit

Cat.#:

E14979r

Brand:
EIAab®
Regulatory Status:
Alternative:

cN-III-like protein

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.312-20ng/mL
Sensitivity:
0.182ng/mL
Specificity:
Natural and recombinant rat Cytosolic 5'-nucleotidase III-like protein
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
-
Rat Nt5c3l ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Recovery
Recovery was determined by spiking various levels of Cytosolic 5'-nucleotidase III-like protein into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

98

92-104

Plasma

100

94-106

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cytosolic 5'-nucleotidase III-like protein and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

102-114%

85-95%

88-98%

98-106%

EDTA plasma(n=5)

100-111%

100-111%

111-120%

103-111%

heparin plasma(n=5)

111-120%

 

100-110%

91-100%

107-115%

 

General Annotation


Sub Unit:
N/A


Function:
Can act both as nucleotidase and as phosphotransferase.


Subcellular Location:
Cytoplasm


This product has not yet been referenced specifically in any publications.
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