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Nt5c3l (GeneName), Cytosolic 5'-nucleotidase III-like protein (ProteinName), 5NT3L_RAT.

Product Name:

Rat Nt5c3l/ Cytosolic 5'-nucleotidase III-like protein ELISA Kit

Cat.#:

E14979r

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

cN-III-like protein

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.312-20ng/mL

Sensitivity:

0.182ng/mL

Specificity:

Natural and recombinant rat Cytosolic 5'-nucleotidase III-like protein

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

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Rat Nt5c3l ELISA Kit

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Product Datasheets

Instruction:

MSDS:

Data
Citations
Sequence / 3D
Feedback Wall (0)

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Recovery

Recovery was determined by spiking various levels of Cytosolic 5'-nucleotidase III-like protein into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	98	92-104
Plasma	100	94-106

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cytosolic 5'-nucleotidase III-like protein and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	102-114%	85-95%	88-98%	98-106%
EDTA plasma(n=5)	100-111%	100-111%	111-120%	103-111%
heparin plasma(n=5)	111-120%	100-110%	91-100%	107-115%