Nt5c3l (GeneName), Cytosolic 5'-nucleotidase III-like protein (ProteinName), 5NT3L_RAT.
Product Name:
Rat Nt5c3l/ Cytosolic 5'-nucleotidase III-like protein ELISA Kit
Cat.#:
E14979r
Brand:
EIAab®
Regulatory Status:
Alternative:
cN-III-like protein
Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.312-20ng/mL
Sensitivity:
0.182ng/mL
Specificity:
Natural and recombinant rat Cytosolic 5'-nucleotidase III-like protein
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:

Research Area:
-
- Data
- Citations
- Sequence / 3D
- Feedback Wall (0)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤5.1%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.5%
Recovery
Recovery was determined by spiking various levels of Cytosolic 5'-nucleotidase III-like protein into serum and plasma.
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
98 |
92-104 |
Plasma |
100 |
94-106 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cytosolic 5'-nucleotidase III-like protein and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
102-114% |
85-95% |
88-98% |
98-106% |
EDTA plasma(n=5) |
100-111% |
100-111% |
111-120% |
103-111% |
heparin plasma(n=5) |
111-120%
|
100-110% |
91-100% |
107-115% |
General Annotation
Sub Unit:
N/A
Function:
Can act both as nucleotidase and as phosphotransferase.
Subcellular Location:
Cytoplasm
This product has not yet been referenced specifically in any publications.
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