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Nt5c3l (GeneName), Cytosolic 5'-nucleotidase III-like protein (ProteinName), 5NT3L_MOUSE.

Product Name:

Mouse Nt5c3l/ Cytosolic 5'-nucleotidase III-like protein ELISA Kit

Cat.#:

E14979m

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

cN-III-like protein

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/ml

Sensitivity:

0.18ng/mL

Specificity:

Natural and recombinant mouse Cytosolic 5'-nucleotidase III-like protein

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

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Mouse Nt5c3l ELISA Kit

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Product Datasheets

Instruction:

MSDS:

Data
Citations
Sequence / 3D
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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤8.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.0%

Recovery

Recovery was determined by spiking various levels of Cytosolic 5'-nucleotidase III-like protein into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	92	86-98
Plasma	94	88-100

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cytosolic 5'-nucleotidase III-like protein and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-105%	94-105%	107-116%	80-90%
EDTA plasma(n=5)	100-109%	110-119%	95-104%	108-117%
heparin plasma(n=5)	94-103%	83-94%	86-95%	112-122%