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Htr2b (基因名), 5-hydroxytryptamine receptor 2B (蛋白名), 5HT2B_MOUSE.

产品名称:

Mouse Htr2b/ 5-hydroxytryptamine receptor 2B ELISA Kit

货号:

商标:

EIAab^®

监管等级:

RUO

别名:

5-HT-2B, 5-HT-2F, NP75 protein, Serotonin receptor 2B

检测方法:

ELISA

特异性:

Natural and recombinant mouse 5-hydroxytryptamine receptor 2B

样品类型:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

样品数据:

研究领域:

规格 & 价格: 登录.

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产品说明书

数据
文献
出版物
序列 / 3D
常见问题解答
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通用注释

亚单元:

Interacts (via C-terminus) with MPDZ.

功能:

G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed:1426253). Also functions as a receptor for various ergot alkaloid derivatives and psychoactive substances (PubMed:1426253, PubMed:16940156). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and downstream signaling cascades and promotes the release of Ca(2+) ions from intracellular stores (By similarity). Plays a role in the regulation of dopamine and 5-hydroxytryptamine release, 5-hydroxytryptamine uptake and in the regulation of extracellular dopamine and 5-hydroxytryptamine levels, and thereby affects neural activity (PubMed:16940156, PubMed:18337424). May play a role in the perception of pain (PubMed:21273425). Plays a role in the regulation of behavior, including impulsive behavior (PubMed:21179162). Required for normal proliferation of embryonic cardiac myocytes and normal heart development (PubMed:10944220, PubMed:11413089). Protects cardiomyocytes against apoptosis (PubMed:12738797). Plays a role in the adaptation of pulmonary arteries to chronic hypoxia (PubMed:12244304). Plays a role in vasoconstriction (PubMed:12244304, PubMed:23346101). Required for normal osteoblast function and proliferation, and for maintaining normal bone density (PubMed:17846081). Required for normal proliferation of the interstitial cells of Cajal in the intestine (PubMed:19941613).

亚细胞位置:

Cell membrane Multi-pass membrane protein Cell junction Synapse Synaptosome

数据库链接

UniGene:

Mm.439747

STRING:

10090.ENSMUSP00000027431

KEGG:

mmu:15559

Pfam:

PF00001

Uniprot:

Q02152

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序列长度:

479

序列:

50:

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450:

479:

TIQSSSIILL | DTLLTENDGD | KAEEQVSYI

3D 模型:

3D 结构:

免疫原序列:

验证序列:

该产品尚未在任何出版物中被引用。

[1].

"Agonist-directed trafficking of signalling at serotonin 5-HT2A, 5-HT2B and 5-HT2C-VSV receptors mediated Gq/11 activation and calcium mobilisation in CHO cells."

(PMID: 18703043)

[2].

"Agonist actions of dihydroergotamine at 5-HT2B and 5-HT2C receptors and their possible relevance to antimigraine efficacy."

(PMID: 12970106)

[3].

"5-HT2B receptor-mediated calcium release from ryanodine-sensitive intracellular stores in human pulmonary artery endothelial cells."

(PMID: 8882600)

[4].

"Cloning and functional characterization of the human 5-HT2B serotonin receptor."

(PMID: 8143856)

[5].

"The human serotonin 5-HT2B receptor: pharmacological link between 5-HT2 and 5-HT1D receptors."

(PMID: 7926008)

[6].

"Crystal Structure of an LSD-Bound Human Serotonin Receptor."

(PMID: 28129538)

[7].

"Structural features for functional selectivity at serotonin receptors."

(PMID: 23519215)

[8].

"Association study of the serotoninergic system in migraine in the Spanish population."

(PMID: 19455600)

[9].

"Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study."

(PMID: 20628086)

[10].

"A population-specific HTR2B stop codon predisposes to severe impulsivity."

(PMID: 21179162)

[1].

小鼠Htr2bELISA试剂盒可以做多少个样本？

小鼠Htr2bELISA试剂盒分为2种规格，96孔和48孔。96孔的试剂盒，标曲和样本都做复孔的话，可以检测40个样本。96孔的试剂盒，标曲和样本都不做复孔的话，可以检测88个样本。

[2].

小鼠Htr2bELISA试剂盒使用视频？

小鼠Htr2bELISA试剂盒实验操作视频在以下网址中，对每一步的实验步骤都做了演示，方便实验员能更好地理解ELISA实验的过程。
https://www.eiaab.com.cn/lesson-tech/805.html

[3].

小鼠Htr2bELISA试剂盒是放在-20℃冰箱保存吗？

EIAab的小鼠Htr2bELISA试剂盒，洗涤液、底物、终止液保存于4℃，其余试剂-20℃冰箱保存。

[4].

小鼠Htr2bELISA试剂盒原理？

双抗体夹心法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本、生物素化抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。
竞争法：用纯化的抗体包被微孔板，制成固相抗体，往包被有固相抗体的微孔中依次加入标准品或受检样本和生物素标记的目标分析物，受检标本中抗原与生物素标记抗原竞争结合有限的抗体。再加入HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。用酶标仪在450nm波长下测定吸光度(OD值）,计算样本浓度。

[5].

小鼠Htr2bELISA试剂盒中需要使用的样品量是多少？

夹心法100μL/孔，竞争法50μL/孔。如样本浓度过高时，应对样本进行稀释，以使稀释后的样本符合试剂盒的检测范围，计算时再乘以相应的稀释倍数。

[6].

如何分析小鼠Htr2bELISA试剂盒数据？

建议标准曲线，并计算样本浓度。对于elisa的曲线拟合，一般建议采用4参数曲线拟合，4参数曲线拟合通常更适合免疫分析。推荐使用专业软件进行曲线拟合，例如curve expert 1.3。根据样本的OD值由标曲查出相应的浓度，再乘以稀释倍数；或用标准物的浓度与OD值计算出标曲的回归方程式，将样本的OD值代入方程式，计算出样本浓度，再乘以稀释倍数，即为样本的实际浓度。以下链接是curve expert 1.3软件拟合曲线的方法。
https://www.eiaab.com.cn/news/502/

[7].

小鼠Htr2bELISA试剂盒中是否包含人和动物的副产物，是否包含感染的或者传染性原料如HIV等？

除了抗体和稀释液中的BSA，不含其它人和动物的副产物，也不含感染材料。

[8].

收集小鼠Htr2bELISA试剂盒血浆样本，用什么作为抗凝剂？

一般建议用EDTA和肝素作为抗凝剂。

[9].

小鼠Htr2bELISA试剂盒酶标板可以拆成几部分？拆的时候是否需要避光，无菌？

小鼠Htr2bELISA试剂盒酶标板是8×12孔条，可拆卸，板子可以拆成12条，注意避免孔污染，不需要避光和无菌。暂时不用的板子，放回原来装的袋子里，密封保存。

[10].

小鼠Htr2bELISA试剂盒样本如何保存？

尽量检测新鲜样本。若无新鲜样本，则4℃保存1周，-20℃保存1个月，-80℃保存2个月。

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