Home > ELISA Kit > Human HTR1B ELISA Kit

HTR1B (GeneName), 5-hydroxytryptamine receptor 1B (ProteinName), 5HT1B_HUMAN.

Product Name:

Human HTR1B/ 5-hydroxytryptamine receptor 1B ELISA Kit

Cat.#:

E10279h

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

5-HT-1B, S12, Serotonin 1D beta receptor, 5-HT-1D-beta, Serotonin receptor 1B, HTR1DB

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

78-5000pg/mL

Sensitivity:

34pg/mL

Specificity:

Natural and recombinant human 5-hydroxytryptamine receptor 1B

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Neurosciences

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Human HTR1B ELISA Kit

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Product Datasheets

Instruction:

MSDS:

Data
Citations
Publication
Sequence / 3D
Feedback Wall (0)

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.5%

Recovery

Recovery was determined by spiking various levels of 5-hydroxytryptamine receptor 1B into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-103
Plasma	99	93-105

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-hydroxytryptamine receptor 1B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	86-96%	109-117%	90-99%	106-118%
EDTA plasma(n=5)	110-120%	109-119%	82-92%	97-109%
heparin plasma(n=5)	92-103%	85-95%	105-105%	83-92%

General Annotation

Sub Unit:

Homodimer. Heterodimer with HTR1D.

Function:

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances, such as lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries.

Subcellular Location:

Cell membrane Multi-pass membrane protein

Database link

UniGene:

Hs.123016

SMR:

P28222

STRING:

9606.ENSP00000358963

KEGG:

hsa:3351

MIM: