HTR1B (GeneName), 5-hydroxytryptamine receptor 1B (ProteinName), 5HT1B_HUMAN.
Product Name:
Human HTR1B/ 5-hydroxytryptamine receptor 1B ELISA Kit
Cat.#:
E10279h
Brand:
EIAab®
Regulatory Status:
Alternative:
5-HT-1B, S12, Serotonin 1D beta receptor, 5-HT-1D-beta, Serotonin receptor 1B, HTR1DB
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
34pg/mL
Specificity:
Natural and recombinant human 5-hydroxytryptamine receptor 1B
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:

Research Area:
Neurosciences
- Data
- Citations
- Publication
- Sequence / 3D
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.6%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤8.5%
Recovery
Recovery was determined by spiking various levels of 5-hydroxytryptamine receptor 1B into serum and plasma.
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
97 |
91-103 |
Plasma |
99 |
93-105 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-hydroxytryptamine receptor 1B and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
86-96% |
109-117% |
90-99% |
106-118% |
EDTA plasma(n=5) |
110-120% |
109-119% |
82-92% |
97-109% |
heparin plasma(n=5) |
92-103%
|
85-95% |
105-105% |
83-92% |
General Annotation
Sub Unit:
Homodimer. Heterodimer with HTR1D.
Function:
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances, such as lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries.
Subcellular Location:
Cell membrane
Multi-pass membrane protein
This product has not yet been referenced specifically in any publications.
[1].
"Lack of association between five serotonin metabolism-related genes and medication overuse headache."
[2].
"[Association study of HTR1A and HTR1B with adolescent idiopathic scoliosis]."
[3].
"Comorbidity between bipolar disorder and alcohol use disorder: association of dopamine and serotonin gene polymorphisms."
[4].
"Fluoxetine response in impulsive-aggressive behavior and serotonin transporter polymorphism in personality disorder."
[5].
"Functional polymorphisms in the serotonin 1B receptor gene (HTR1B) predict self-reported anger and hostility among young men."
[7].
"An association study of the serotonin transporter and receptor genes with the suicidal ideation of major depression in a Chinese Han population."
[8].
"Association analysis of serotonin receptor 1B (HTR1B) and brain-derived neurotrophic factor gene polymorphisms in Borderline personality disorder."
[9].
"The relationship between serotonin receptor 1B polymorphisms A-161T and alcohol dependence."
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