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首页  >  酶联免疫试剂盒  >  Mouse Eif4ebp1 ELISA Kit
Eif4ebp1 (基因名), Eukaryotic translation initiation factor 4E-binding protein 1 (蛋白名), 4EBP1_MOUSE.
产品名称:

Mouse Eif4ebp1/ Eukaryotic translation initiation factor 4E-binding protein 1 ELISA Kit
4E-BP1

货号:

E2693m

商标:
EIAab®
监管等级:
别名:

4E-BP1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I

检测方法:
ELISA
实验类型:
Sandwich
检测范围:
0.156-10ng/ml
灵敏度:
0.078ng/mL
特异性:
Natural and recombinant mouse Eukaryotic translation initiation factor 4E-binding protein 1
样品类型:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
样品数据:
实验步骤:
实验步骤
研究领域:
Cancer
Mouse Eif4ebp1 ELISA Kit
规格 & 价格: cart
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Mouse Eif4ebp1 ELISA Kit
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产品说明书
数据表: 下载说明书
说明书: 下载说明书
MSDS: MSDS


精密度
批内差:已知浓度的3个样本在一个板子内重复检测20次,以评估批内精密度。
批内 CV: ≤8.1%
批间差:已知浓度的3个样本在不同的板子上重复测定5次,以评估测定批间精密度。
批间 CV: ≤10.1%
回收率
回收率:低、中和高浓度的分析物被掺入到血清或者血浆样本中,进行回收实验测定。

Sample Type

Average(%)

Recovery Range(%)

Serum

90

84-96

Plasma

92

86-98

 

 

 

 

线性
线性:给定样本通过梯度稀释,每次稀释的测量值与理论值的比值。

Sample

1:2

1:4

1:8

1:16

serum(n=5)

98-106%

87-96%

98-108%

92-104%

EDTA plasma(n=5)

92-101%

102-113%

103-113%

84-94%

heparin plasma(n=5)

82-91%

 

81-91%

114-124%

98-108%

 

通用注释


亚单元:
Hypophosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation (PubMed:7629182). Interacts (via TOS motif) with RPTOR; promoting phosphorylation by mTORC1 (PubMed:24139800).


功能:
Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation (By similarity). Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways (PubMed:7629182).


亚细胞位置:
N/A


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