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Hibadh (GeneName), 3-hydroxyisobutyrate dehydrogenase, mitochondrial (ProteinName), 3HIDH_MOUSE.
Product Name:

Mouse Hibadh/ 3-hydroxyisobutyrate dehydrogenase, mitochondrial ELISA Kit

Cat.#:

E12565m

Brand:
EIAab®
Regulatory Status:
Alternative:

HIBADH

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.081ng/mL
Specificity:
Natural and recombinant mouse 3-hydroxyisobutyrate dehydrogenase, mitochondrial
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Cell Biology
Mouse Hibadh ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Recovery
Recovery was determined by spiking various levels of 3-hydroxyisobutyrate dehydrogenase, mitochondrial into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

100

94-106

Plasma

102

96-108

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 3-hydroxyisobutyrate dehydrogenase, mitochondrial and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

87-99%

97-107%

97-107%

110-119%

EDTA plasma(n=5)

110-120%

89-99%

106-116%

108-120%

heparin plasma(n=5)

95-105%

 

92-101%

98-107%

91-103%

 

General Annotation


Sub Unit:
Homodimer.


Function:
N/A


Subcellular Location:
Mitochondrion


This product has not yet been referenced specifically in any publications.

[1].
"Clinical, biochemical, and molecular findings in three patients with 3-hydroxyisobutyric aciduria."

[2].
"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."

[3].
"Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging."

[4].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."

[5].
"The DNA sequence of human chromosome 7."

[6].
"Human liver protein map: update 1993."

[7].
"Architecture of the human interactome defines protein communities and disease networks."

[8].
"Panorama of ancient metazoan macromolecular complexes."

[9].
"N-terminome analysis of the human mitochondrial proteome."

[10].
"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
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