HIBADH (GeneName), 3-hydroxyisobutyrate dehydrogenase, mitochondrial (ProteinName), 3HIDH_HUMAN.
Product Name:
Human HIBADH/ 3-hydroxyisobutyrate dehydrogenase, mitochondrial ELISA Kit
Cat.#:
E12565h
Brand:
EIAab®
Regulatory Status:
Alternative:
HIBADH
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.08ng/mL
Specificity:
Natural and recombinant human 3-hydroxyisobutyrate dehydrogenase, mitochondrial
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Cell Biology
- Data
- Citations
- Publication
- Sequence / 3D
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.5%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤5.9%
Recovery
Recovery was determined by spiking various levels of 3-hydroxyisobutyrate dehydrogenase, mitochondrial into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
90 |
84-96 |
|
Plasma |
92 |
86-98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 3-hydroxyisobutyrate dehydrogenase, mitochondrial and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
99-109% |
102-112% |
97-110% |
104-112% |
|
EDTA plasma(n=5) |
107-119% |
99-109% |
107-116% |
92-104% |
|
heparin plasma(n=5) |
106-116%
|
105-114% |
104-116% |
95-105% |
General Annotation
Sub Unit:
Homodimer.
Function:
N/A
Subcellular Location:
Mitochondrion
Database link
Valid Sequence:
This product has not yet been referenced specifically in any publications.
[1].
"Clinical, biochemical, and molecular findings in three patients with 3-hydroxyisobutyric aciduria."
[2].
"Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression."
[3].
"Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging."
[4].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
[7].
"Architecture of the human interactome defines protein communities and disease networks."
[10].
"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
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