Haao (GeneName), 3-hydroxyanthranilate 3,4-dioxygenase (ProteinName), 3HAO_RAT.
Product Name:
Rat Haao/ 3-hydroxyanthranilate 3,4-dioxygenase ELISA Kit
Cat.#:
E10868r
Brand:
EIAab®
Regulatory Status:
Alternative:
3-hydroxyanthranilate oxygenase, 3-HAO, 3-hydroxyanthranilic acid dioxygenase, HAD
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.114ng/mL
Specificity:
Natural and recombinant rat 3-hydroxyanthranilate 3,4-dioxygenase
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Neurosciences
- Data
- Citations
- Publication
- Sequence / 3D
- Feedback Wall (0)
Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.6%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤5.2%
Recovery
Recovery was determined by spiking various levels of 3-hydroxyanthranilate 3,4-dioxygenase into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
98 |
92-104 |
|
Plasma |
100 |
94-106 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 3-hydroxyanthranilate 3,4-dioxygenase and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
103-111% |
94-104% |
104-116% |
106-115% |
|
EDTA plasma(n=5) |
97-106% |
117-127% |
92-102% |
97-108% |
|
heparin plasma(n=5) |
89-98%
|
107-117% |
110-119% |
96-106% |
General Annotation
Sub Unit:
Monomer.
Function:
Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.
Subcellular Location:
Cytoplasm
Database link
Valid Sequence:
This product has not yet been referenced specifically in any publications.
[1].
"Molecular cloning and functional expression of human 3-hydroxyanthranilic-acid dioxygenase."
[3].
"Crystal structures of human 3-hydroxyanthranilate 3,4-dioxygenase with native and non-native metals bound in the active site."
[4].
"Evidence for genes on chromosome 2 contributing to alcohol dependence with conduct disorder and suicide attempts."
[5].
"Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling."
[6].
"Identification of candidate epigenetic biomarkers for ovarian cancer detection."
[7].
"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
[8].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
[9].
"Cloning of human 3-hydroxyanthranilic acid dioxygenase in Escherichia coli: characterisation of the purified enzyme and its in vitro inhibition by Zn2+."
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