Hsd3b1 (GeneName), 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (ProteinName), 3BHS1_MOUSE.
Product Name:
Mouse Hsd3b1/ 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 ELISA Kit
Cat.#:
E2793m
Brand:
EIAab®
Regulatory Status:
Alternative:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, Hsd3b, 3-beta-hydroxy-5-ene steroid dehydrogenase, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3-beta-hydroxysteroid 3-dehydrogenase, Delta-5-3-ketosteroid isomerase, Dihydrotestosterone oxidoreductase, Steroid Delta-isomerase
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
39pg/mL
Specificity:
Natural and recombinant mouse 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:

Research Area:
Metabolism
- Data
- Citations
- Publication
- Sequence / 3D
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤8.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤10.2%
Recovery
Recovery was determined by spiking various levels of 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 into serum and plasma.
Sample Type |
Average(%) |
Recovery Range(%) |
Serum |
101 |
95-107 |
Plasma |
103 |
97-109 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
1:16 |
serum(n=5) |
104-114% |
95-103% |
104-114% |
87-95% |
EDTA plasma(n=5) |
110-119% |
110-120% |
95-104% |
97-108% |
heparin plasma(n=5) |
100-109%
|
104-117% |
102-112% |
98-108% |
General Annotation
Sub Unit:
N/A
Function:
A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD(+) as preferred electron donor for the 3-beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity.
Subcellular Location:
Endoplasmic reticulum membrane
Single-pass membrane protein
Mitochondrion membrane
Single-pass membrane protein
Database link
This product has not yet been referenced specifically in any publications.
[1].
"Single and multigenic analysis of the association between variants in 12 steroid hormone metabolism genes and risk of prostate cancer."
[2].
"Steroid biosynthesis and renal excretion in human essential hypertension: association with blood pressure and endogenous ouabain."
[3].
"Evaluation of genetic variations in the androgen and estrogen metabolic pathways as risk factors for sporadic and familial prostate cancer."
[4].
"The hormonal phenotype of Nonclassic 3 beta-hydroxysteroid dehydrogenase (HSD3B) deficiency in hyperandrogenic females is associated with insulin-resistant polycystic ovary syndrome and is not a variant of inherited HSD3B2 deficiency."
[5].
"Joint effect of HSD3B1 and HSD3B2 genes is associated with hereditary and sporadic prostate cancer susceptibility."
[6].
"Molecular cloning and expression of human trophoblast antigen FDO161G and its identification as 3 beta-hydroxy-5-ene steroid dehydrogenase."
[7].
"Full length cDNA structure and deduced amino acid sequence of human 3 beta-hydroxy-5-ene steroid dehydrogenase."
[9].
"Genetic variation in the estrogen metabolic pathway and mammographic density as an intermediate phenotype of breast cancer."
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