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Hsd3b1 (GeneName), 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (ProteinName), 3BHS1_MOUSE.

Product Name:

Mouse Hsd3b1/ 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 ELISA Kit

Cat.#:

E2793m

Brand:

EIAab^®

Regulatory Status:

RUO

Alternative:

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, Hsd3b, 3-beta-hydroxy-5-ene steroid dehydrogenase, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3-beta-hydroxysteroid 3-dehydrogenase, Delta-5-3-ketosteroid isomerase, Dihydrotestosterone oxidoreductase, Steroid Delta-isomerase

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

78-5000pg/mL

Sensitivity:

39pg/mL

Specificity:

Natural and recombinant mouse 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

Sample Type:

Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Sample Data:

Assay Procedure:

Research Area:

Metabolism

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Mouse Hsd3b1 ELISA Kit

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Product Datasheets

Datasheet:

Instruction:

MSDS:

Data
Citations
Publication
Sequence / 3D
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Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤8.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.2%

Recovery

Recovery was determined by spiking various levels of 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 into serum and plasma.

Sample Type	Average(%)	Recovery Range(%)
Serum	101	95-107
Plasma	103	97-109

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	104-114%	95-103%	104-114%	87-95%
EDTA plasma(n=5)	110-119%	110-120%	95-104%	97-108%
heparin plasma(n=5)	100-109%	104-117%	102-112%	98-108%

General Annotation

Sub Unit:

N/A

Function:

A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD(+) as preferred electron donor for the 3-beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity.

Subcellular Location:

Endoplasmic reticulum membrane Single-pass membrane protein Mitochondrion membrane Single-pass membrane protein

Database link

UniGene:

Mm.140811

STRING:

10090.ENSMUSP00000102630

KEGG:

mmu:15492

Pfam:

PF01073

Uniprot:

P24815

Related Product

Human HSD3B1 ELISA Kit

Rat Hsd3b1 ELISA Kit

Human HSD3B1 CLIA Kit

Rat Hsd3b1 CLIA Kit

Human HSD3B1 Protein

Sequence length:

373

Sequence:

50:

100:

150:

200:

250:

300:

350:

379:

WEEAKQKTSE | WIGTI VEQH | REILDTKCQ

3D Structure:

Predicted Epitope:

Valid Sequence:

This product has not yet been referenced specifically in any publications.

[1].

"Single and multigenic analysis of the association between variants in 12 steroid hormone metabolism genes and risk of prostate cancer."

(PMID: 19505920)

[2].

"Steroid biosynthesis and renal excretion in human essential hypertension: association with blood pressure and endogenous ouabain."

(PMID: 19197249)

[3].

"Evaluation of genetic variations in the androgen and estrogen metabolic pathways as risk factors for sporadic and familial prostate cancer."

(PMID: 17507624)

[4].

"The hormonal phenotype of Nonclassic 3 beta-hydroxysteroid dehydrogenase (HSD3B) deficiency in hyperandrogenic females is associated with insulin-resistant polycystic ovary syndrome and is not a variant of inherited HSD3B2 deficiency."

(PMID: 14764797)

[5].

"Joint effect of HSD3B1 and HSD3B2 genes is associated with hereditary and sporadic prostate cancer susceptibility."

(PMID: 11912155)

[6].

"Molecular cloning and expression of human trophoblast antigen FDO161G and its identification as 3 beta-hydroxy-5-ene steroid dehydrogenase."

(PMID: 1920284)

[7].

"Full length cDNA structure and deduced amino acid sequence of human 3 beta-hydroxy-5-ene steroid dehydrogenase."

(PMID: 2779585)

[8].

"Genetic susceptibility to distinct bladder cancer subphenotypes."