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HSD3B1 (GeneName), 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (ProteinName), 3BHS1_HUMAN.
Product Name:

Human HSD3B1/ 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 ELISA Kit

Cat.#:

E2793h

Brand:
EIAab®
Regulatory Status:
Alternative:

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3-beta-HSD I, 3BH, HSDB3A, 3-beta-hydroxy-5-ene steroid dehydrogenase, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3-beta-hydroxysteroid 3-dehydrogenase, Delta-5-3-ketosteroid isomerase, Dihydrotestosterone oxidoreductase, Steroid Delta-isomerase, Trophoblast antigen FDO161G

Detection Method:
ELISA
Assay Type:
Competitive
Detection Range:
0.312-20ng/mL
Sensitivity:
0.19ng/mL
Specificity:
Natural and recombinant human 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Assay Procedure
Research Area:
Metabolism
Human HSD3B1 ELISA Kit
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Human HSD3B1 ELISA Kit
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Product Datasheets
Instruction: Down Instruction
MSDS: MSDS


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

Recovery
Recovery was determined by spiking various levels of 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

95

89-101

Plasma

97

91-103

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

85-95%

86-98%

113-123%

80-90%

EDTA plasma(n=5)

110-120%

88-98%

108-118%

115-124%

heparin plasma(n=5)

99-109%

 

101-101%

105-116%

114-124%

 

General Annotation


Sub Unit:
N/A


Function:
A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, 17alpha-hydroxypregnenolone to 17alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone (PubMed:1401999, PubMed:2139411). Expected to use NAD(+) as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable).


Subcellular Location:
Endoplasmic reticulum membrane Single-pass membrane protein Mitochondrion membrane Single-pass membrane protein


This product has not yet been referenced specifically in any publications.

[1].
"Single and multigenic analysis of the association between variants in 12 steroid hormone metabolism genes and risk of prostate cancer."

[2].
"Steroid biosynthesis and renal excretion in human essential hypertension: association with blood pressure and endogenous ouabain."

[3].
"Evaluation of genetic variations in the androgen and estrogen metabolic pathways as risk factors for sporadic and familial prostate cancer."

[4].
"The hormonal phenotype of Nonclassic 3 beta-hydroxysteroid dehydrogenase (HSD3B) deficiency in hyperandrogenic females is associated with insulin-resistant polycystic ovary syndrome and is not a variant of inherited HSD3B2 deficiency."

[5].
"Joint effect of HSD3B1 and HSD3B2 genes is associated with hereditary and sporadic prostate cancer susceptibility."

[6].
"Molecular cloning and expression of human trophoblast antigen FDO161G and its identification as 3 beta-hydroxy-5-ene steroid dehydrogenase."

[7].
"Full length cDNA structure and deduced amino acid sequence of human 3 beta-hydroxy-5-ene steroid dehydrogenase."

[8].
"Genetic susceptibility to distinct bladder cancer subphenotypes."

[9].
"Genetic variation in the estrogen metabolic pathway and mammographic density as an intermediate phenotype of breast cancer."

[10].
"A Large-scale genetic association study of esophageal adenocarcinoma risk."
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