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PPP2R1A (GeneName), Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (ProteinName), 2AAA_HUMAN.
Product Name:

Human PPP2R1A/ Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform ELISA Kit

 
Cat.#:
-
Brand:
EIAab®
Regulatory Status:
Alternative:

Medium tumor antigen-associated 61 kDa protein,PP2A subunit A isoform PR65-alpha,PP2A subunit A isoform R1-alpha

Detection Method:
ELISA
Specificity:
Natural and recombinant human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Research Area:
Cancer
Product Overview:
is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform. The ELISA analytical biochemical technique of the kit is based on Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform antibody-Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Human PPP2R1A ELISA Kit
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Product Datasheets
Datasheet:


General Annotation


Sub Unit:
Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with FOXO1; the interaction dephosphorylates FOXO1 on AKT-mediated phosphorylation sites (By similarity). PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGO1. Interacts with PLA2G16; this interaction might decrease PP2A activity. Interacts with CTTNBP2NL.


Function:
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis.


Subcellular Location:
Cytoplasm Chromosome Centromere Centromeric localization requires the presence of BUB1.


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