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Sfn (GeneName), 14-3-3 protein sigma (ProteinName), 1433s_mouse.
Product Name:

Mouse Sfn/ 14-3-3 protein sigma ELISA Kit

 
Cat.#:

E15106m

Brand:
EIAab®
Regulatory Status:
Alternative:

Stratifin,Mkrn3

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
31.2-2000pg/mL
Sensitivity:
15.13pg/mL
Specificity:
Natural and recombinant mouse 14-3-3 protein sigma
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Cancer
Product Overview:
E15106m is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (14-3-3 protein sigma) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 14-3-3 protein sigma. The ELISA analytical biochemical technique of the E15106m kit is based on 14-3-3 protein sigma antibody-14-3-3 protein sigma antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 14-3-3 protein sigma antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 14-3-3 protein sigma. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Mouse Sfn ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: 0

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: 0

Recovery
Recovery was determined by spiking various levels of 14-3-3 protein sigma into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

80

80-80

Plasma

80

80-80

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 14-3-3 protein sigma and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

102-114%

103-113%

110-120%

107-118%

EDTA plasma(n=5)

99-108%

116-125%

83-91%

97-107%

heparin plasma(n=5)

92-101%

 

100-109%

102-111%

106-116%

 

General Annotation


Sub Unit:
Homodimer. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29 and VPS35. Interacts with GAB2 (By similarity). Interacts with KRT17. Interacts with SAMSN1. Interacts with SRPK2 (By similarity). Interacts with COPS6 (By similarity). Interacts with RFWD2; this interaction leads to proteasomal degradation (By similarity).Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB (By similarity). Interacts with SLITRK1.


Function:
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.


Subcellular Location:
Cytoplasm Nucleus Secreted May be secreted by a non-classical secretory pathway.


This product has not yet been referenced specifically in any publications.
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