Ywhaz (GeneName), 14-3-3 protein zeta/delta (ProteinName), 1433Z_RAT.
Product Name:
Rat Ywhaz/ 14-3-3 protein zeta/delta ELISA Kit
Cat.#:
E14294r
Brand:
EIAab®
Regulatory Status:
Alternative:
Mitochondrial import stimulation factor S1 subunit,Protein kinase C inhibitor protein 1,KCIP-1,Msfs1
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
31.2-2000pg/mL
Sensitivity:
15.68pg/mL
Specificity:
Natural and recombinant rat 14-3-3 protein zeta/delta
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Neurosciences
Product Overview:
E14294r is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay)
Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (14-3-3 protein zeta/delta) ELISA Kit target analytes in biological samples. The concentration gradients of
the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 14-3-3 protein zeta/delta. The ELISA analytical biochemical
technique of the E14294r kit is based on 14-3-3 protein zeta/delta antibody-14-3-3 protein zeta/delta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 14-3-3 protein zeta/delta
antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 14-3-3 protein zeta/delta. Appropriate sample types may include undiluted human body fluids
and/or tissue homogenates,
secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.1%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.4%
Recovery
Recovery was determined by spiking various levels of 14-3-3 protein zeta/delta into serum and plasma.
|
Sample Type |
Average(%) |
Recovery Range(%) |
|
Serum |
100 |
94-106 |
|
Plasma |
102 |
96-108 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 14-3-3 protein zeta/delta and their serial dilutions.
The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1：2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
97-106% |
107-117% |
85-97% |
101-111% |
|
EDTA plasma(n=5) |
83-91% |
88-98% |
104-113% |
104-115% |
|
heparin plasma(n=5) |
95-95%
|
86-97% |
109-120% |
107-117% |
General Annotation
Sub Unit:
Homodimer. Heterodimerizes with YWHAE (By similarity). Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58 (By similarity). Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with CDK16 and with WEE1 (C-terminal). Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with Thr-phosphorylated ITGB2. Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation. It may also prevent thiol-dependent inactivation. Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization (By similarity). Interacts with GAB2 and SAMSN1. Binds to TLK2 (By similarity). Interacts with BSPRY. Interacts with BCL2L11. Interacts with the 'Thr-369' phosphorylated form of DAPK2 (By similarity). Interacts with PI4KB, TBC1D22A and TBC1D22B (By similarity). Interacts with ZFP36L1 (via phosphorylated form); this interaction occurs in a p38 MAPK- and AKT-signaling pathways (By similarity). Interacts with SLITRK1.
Function:
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Subcellular Location:
Cytoplasm
Melanosome
Located to stage I to stage IV melanosomes.
Database links
Vaild Sequence:
This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
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Publishing research using this product ?
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[1].
"ACBD3 interaction with TBC1 domain 22 protein is differentially affected by enteroviral and kobuviral 3A protein binding."
[2].
"Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score."
[4].
"Conduct disorder and ADHD: evaluation of conduct problems as a categorical and quantitative trait in the international multicentre ADHD genetics study."
[5].
"Phosphorylation-dependent binding of 14-3-3 terminates signalling by the Gab2 docking protein."
[7].
"Gene-gene interaction between 14-3-3 zeta and butyrylcholinesterase modulates Alzheimer's disease risk."
[8].
"Protein kinase A phosphorylates and regulates dimerization of 14-3-3 epsilon."
[9].
"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes."
[10].
"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
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