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Ywhaz (GeneName), 14-3-3 protein zeta/delta (ProteinName), 1433Z_MOUSE.
Product Name:

Mouse Ywhaz/ 14-3-3 protein zeta/delta ELISA Kit

 
Cat.#:

E14294m

Brand:
EIAab®
Regulatory Status:
Alternative:

Protein kinase C inhibitor protein 1,KCIP-1,SEZ-2

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78-5000pg/mL
Sensitivity:
39pg/mL
Specificity:
Natural and recombinant mouse 14-3-3 protein zeta/delta
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Sample Data:
Assay Procedure:
Research Area:
Neurosciences
Product Overview:
E14294m is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A Disintegrin and Metalloprotease 30 (14-3-3 protein zeta/delta) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 14-3-3 protein zeta/delta. The ELISA analytical biochemical technique of the E14294m kit is based on 14-3-3 protein zeta/delta antibody-14-3-3 protein zeta/delta antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 14-3-3 protein zeta/delta antigen targets in samples. The ELISA Kit is designed to detect native, recombinant, 14-3-3 protein zeta/delta. Appropriate sample types may include undiluted human body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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Mouse Ywhaz ELISA Kit
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Product Datasheets
Datasheet:
Instruction:
MSDS:


Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤8.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.1%

Recovery
Recovery was determined by spiking various levels of 14-3-3 protein zeta/delta into serum and plasma.

Sample Type

Average(%)

Recovery Range(%)

Serum

83

80-89

Plasma

85

80-91

 

 

 

 

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 14-3-3 protein zeta/delta and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

1:16

serum(n=5)

106-116%

109-118%

102-114%

88-98%

EDTA plasma(n=5)

106-118%

94-107%

104-114%

102-112%

heparin plasma(n=5)

110-119%

 

103-113%

110-122%

111-120%

 

General Annotation


Sub Unit:
Homodimer. Heterodimerizes with YWHAE (By similarity). Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58 (By similarity). Interacts with FOXO4, NOXA1, SSH1 and ARHGEF2. Interacts with CDK16 and with WEE1 (C-terminal). Interacts with MLF1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with BSPRY. Interacts with Thr-phosphorylated ITGB2 (By similarity). Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form). Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions, MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional activity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form); the interaction retains ABL1 in the cytoplasm. Interacts with PKA-phosphorylated AANAT; the interaction modulates AANAT enzymatic activity by increasing affinity for arylalkylamines and acetyl-CoA and protecting the enzyme from dephosphorylation and proteasomal degradation (By similarity). It may also prevent thiol-dependent inactivation (By similarity). Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization (By similarity). Interacts with GAB2 (By similarity). Interacts with SAMSN1. Interacts with BCL2L11 and TLK2. Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B (By similarity). Interacts with ZFP36L1 (via phosphorylated form); this interaction occurs in a p38 MAPK- and AKT-signaling pathways (PubMed:22701344). Interacts with SLITRK1.


Function:
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.


Subcellular Location:
Cytoplasm Melanosome Located to stage I to stage IV melanosomes.


This product has not yet been referenced specifically in any publications.
Publishing research using this product ?
Please let us know and we will provide a credit for your next order.

[1].
"ACBD3 interaction with TBC1 domain 22 protein is differentially affected by enteroviral and kobuviral 3A protein binding."

[2].
"Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score."

[3].
"Examination of association of genes in the serotonin system to autism."

[4].
"Conduct disorder and ADHD: evaluation of conduct problems as a categorical and quantitative trait in the international multicentre ADHD genetics study."

[5].
"Phosphorylation-dependent binding of 14-3-3 terminates signalling by the Gab2 docking protein."

[6].
"14-3-3 zeta and tau genes interactively decrease Alzheimer's disease risk."

[7].
"Gene-gene interaction between 14-3-3 zeta and butyrylcholinesterase modulates Alzheimer's disease risk."

[8].
"Protein kinase A phosphorylates and regulates dimerization of 14-3-3 epsilon."

[9].
"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes."

[10].
"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
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